Zhonghua Jie He He Hu Xi Za Zhi. 2003 Jun;26(6):339-42.
To evaluate the significance of serum specific antibodies against severe acute respiratory syndrome (SARS) virus in the diagnosis of SARS and the concordance of IFA and ELISA in the antibodies measurements.
Specific antibodies of IgM and IgG against SARS virus in 267 serum specimens from patients with SARS and 132 from non-SARS patients or healthy persons were assayed with IFA or ELISA. The sensitivity, specificity, positive predictive value (ppv), negative predictive value (NPV), and accuracy were used to evaluate the significance of the antibodies in diagnosis of SARS. The concordance of different measurements was tested with Kappa.
The positive rate of specific IgM and IgG detected by IFA increased sharply on the eleventh day of the disease. The sensitivity, specificity, PPV, NPV, and accuracy of IgM in diagnosis of SARS on eleventh day or later were 65.6%, 100.0%, 100.0%, 71.0% and 81.3%, respectively, and IgG were 91.1%, 97.0% 97.3%, 90.1%, and 93.8%, respectively. Similar results could be seen when IgM and IgG were detected by ELISA. The Kappa of IFA and ELISA for IgM and IgG were 0.640 and 0.779 respectively.
The antibodies against SARS virus can be detected in the mid-and late stage of SARS. The antibodies can be used in the serological diagnosis of SARS. There is high concordance of IFA and ELISA results in measurement of SARS antibodies.
评估血清中严重急性呼吸综合征（SARS）病毒特异性抗体在SARS诊断中的意义以及免疫荧光分析（IFA）和酶联免疫吸附测定（ELISA）在抗体检测中的一致性。
采用IFA或ELISA检测267例SARS患者血清标本以及132例非SARS患者或健康人血清标本中针对SARS病毒的IgM和IgG特异性抗体。用敏感性、特异性、阳性预测值（PPV）、阴性预测值（NPV）和准确性评估这些抗体在SARS诊断中的意义。用Kappa检验不同检测方法结果的一致性。
IFA检测的特异性IgM和IgG阳性率在发病第11天急剧上升。发病第11天及以后，IgM诊断SARS的敏感性、特异性、PPV、NPV和准确性分别为65.6%、100.0%、100.0%、71.0%和81.3%，IgG分别为91.1%、97.0%、97.3%、90.1%和93.8%。ELISA检测IgM和IgG时可见类似结果。IFA和ELISA检测IgM和IgG的Kappa值分别为0.640和0.779。
SARS病毒抗体在SARS的中晚期可被检测到。这些抗体可用于SARS的血清学诊断。IFA和ELISA检测SARS抗体的结果具有高度一致性。
