工程改造嗜热栖热放线菌用于高温放射性废物环境的生物修复。
Engineering Deinococcus geothermalis for bioremediation of high-temperature radioactive waste environments.
作者信息
Brim Hassan, Venkateswaran Amudhan, Kostandarithes Heather M, Fredrickson James K, Daly Michael J
机构信息
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA.
出版信息
Appl Environ Microbiol. 2003 Aug;69(8):4575-82. doi: 10.1128/AEM.69.8.4575-4582.2003.
Abstract
Deinococcus geothermalis is an extremely radiation-resistant thermophilic bacterium closely related to the mesophile Deinococcus radiodurans, which is being engineered for in situ bioremediation of radioactive wastes. We report that D. geothermalis is transformable with plasmids designed for D. radiodurans and have generated a Hg(II)-resistant D. geothermalis strain capable of reducing Hg(II) at elevated temperatures and in the presence of 50 Gy/h. Additionally, D. geothermalis is capable of reducing Fe(III)-nitrilotriacetic acid, U(VI), and Cr(VI). These characteristics support the prospective development of this thermophilic radiophile for bioremediation of radioactive mixed waste environments with temperatures as high as 55 degrees C.
摘要
嗜热栖热放线菌是一种具有极强辐射抗性的嗜热细菌，与嗜温的耐辐射奇异球菌密切相关，后者正被改造用于放射性废物的原位生物修复。我们报告称，嗜热栖热放线菌可被用为耐辐射奇异球菌设计的质粒转化，并已培育出一种耐汞（II）的嗜热栖热放线菌菌株，该菌株能够在高温及50戈瑞/小时的条件下还原汞（II）。此外，嗜热栖热放线菌能够还原次氮基三乙酸铁（III）、铀（VI）和铬（VI）。这些特性支持了这种嗜热嗜辐射菌在高达55摄氏度的放射性混合废物环境生物修复方面的潜在发展。
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