Saavedra F E, Lebedeva A I, Solveĭ N V
Antibiotiki. 1975 Oct;20(10):926-9.
The method of back turbidimetry was used for determination of the biological activity of the antibiotics, since high turbidity of the nutrient medium with Staph. aureus as the testculture prevented from direct measurements. Broth containing phosphate buffer, Staph. aureus and definite concentrations of the antibiotic was used as the reference solution. The experiments showed that the differences in the biological activities of tetracycline hydrochloride and morphocycline may be found with the method of back turbidimetry 6-8 hours after the microbe cultivation on media with the antibiotics.
由于以金黄色葡萄球菌作为测试培养物时营养培养基的高浊度妨碍了直接测量，因此采用反向比浊法来测定抗生素的生物活性。含有磷酸盐缓冲液、金黄色葡萄球菌和一定浓度抗生素的肉汤用作参比溶液。实验表明，在含有抗生素的培养基上培养微生物6-8小时后，采用反向比浊法可以发现盐酸四环素和吗啉环素生物活性的差异。
