通过修饰的DNA连接多个偶氮苯对SP6 RNA聚合酶转录反应进行有效的光调节。

Effective photo-regulation of transcription reaction by SP6 RNA polymerase with modified DNA tethering multiple azobenzenes.

作者信息

Liu Mingzhe, Asanuma Hiroyuki, Komiyama Makoto

机构信息

Research Center for Advanced Science and Technology, The University of Tokyo, 4-6-1, Komaba, Meguro-ku, Tokyo 153-8904, Japan.

出版信息

Nucleic Acids Res Suppl. 2003(3):265-6. doi: 10.1093/nass/3.1.265.

DOI:10.1093/nass/3.1.265

PMID:14510482

Abstract

Effective photo-regulation of transcription reaction by SP6 RNA polymerase (RNAP) was achieved with photo-responsive SP6 promoter tethering two azobenzenes. With one azobenzene in either TATA or RNAP binding region of the SP6 promoter, photo-regulation activity was very small. But when two azobenzenes were introduced into both regions, efficient photo-regulation of transcription was attained: transcription proceeded 3.5 fold faster under UV irradiation than under dark.

摘要

通过将两个偶氮苯连接到光响应性SP6启动子上，实现了SP6 RNA聚合酶（RNAP）对转录反应的有效光调节。在SP6启动子的TATA或RNAP结合区域中含有一个偶氮苯时，光调节活性非常小。但是当在两个区域都引入两个偶氮苯时，就实现了对转录的有效光调节：在紫外线照射下转录速度比在黑暗中快3.5倍。