脊椎动物和蚊子样本中检测西尼罗河病毒的病毒检测方案。
Virus detection protocols for west nile virus in vertebrate and mosquito specimens.
作者信息
Kauffman Elizabeth B, Jones Susan A, Dupuis Alan P, Ngo Kiet A, Bernard Kristen A, Kramer Laura D
机构信息
The Arbovirus Laboratories, Wadsworth Center, New York State Department of Health, Slingerlands, New York 12159, USA.
出版信息
J Clin Microbiol. 2003 Aug;41(8):3661-7. doi: 10.1128/JCM.41.8.3661-3667.2003.
Abstract
The recent outbreaks of West Nile virus (WNV) infection in the northeastern United States and other regions of the world have made it essential to develop efficient, sensitive, and rapid protocols for virus surveillance. Laboratory testing is the backbone of any surveillance program. Protocols to detect the presence of WNV have been refined since 1999 for sensitivity, speed, efficiency, and specificity. This paper presents the protocols currently used by the New York State Department of Health to handle vertebrate and mosquito specimens that have been submitted for WNV testing to the Arbovirus Laboratories of the Wadsworth Center.
摘要
近期美国东北部及世界其他地区爆发的西尼罗河病毒（WNV）感染疫情，使得制定高效、灵敏且快速的病毒监测方案变得至关重要。实验室检测是任何监测计划的核心。自1999年以来，用于检测WNV存在的方案在灵敏度、速度、效率和特异性方面都得到了改进。本文介绍了纽约州卫生部目前用于处理提交至沃兹沃思中心虫媒病毒实验室进行WNV检测的脊椎动物和蚊子标本的方案。
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Emerg Infect Dis. 2002 Sep;8(9):979-82. doi: 10.3201/eid0809.020152.
2
West Nile Virus activity--United States, 2001.西尼罗河病毒活动情况——美国，2001年
MMWR Morb Mortal Wkly Rep. 2002 Jun 14;51(23):497-501.
3
Real-time PCR in virology.病毒学中的实时聚合酶链反应
Nucleic Acids Res. 2002 Mar 15;30(6):1292-305. doi: 10.1093/nar/30.6.1292.
4
West Nile virus infection in birds and mosquitoes, New York State, 2000.2000年纽约州鸟类和蚊子中的西尼罗河病毒感染
Emerg Infect Dis. 2001 Jul-Aug;7(4):679-85. doi: 10.3201/eid0704.010415.
5
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J Virol Methods. 2001 May;94(1-2):121-8. doi: 10.1016/s0166-0934(01)00279-8.
7
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