登革病毒1型变体分子检测方法的改进
Improved molecular detection of dengue virus serotype 1 variants.
作者信息
Reynes Jean-Marc, Ong Sivuth, Mey Channa, Ngan Chantha, Hoyer Stefan, Sall Amadou A
机构信息
Institut Pasteur du Cambodge. National Dengue Control Programme, Ministry of Health. World Health Organization, Phnom Penh, Cambodia.
出版信息
J Clin Microbiol. 2003 Aug;41(8):3864-7. doi: 10.1128/JCM.41.8.3864-3867.2003.
Abstract
The dengue virus molecular typing method described by Lanciotti and coworkers (R. S. Lanciotti, C. H. Calisher, D. J. Gubler, G. J. Chang, and A. Vance-Vorndam, J. Clin. Microbiol. 30:545-551, 1992) is used worldwide for diagnosis and surveillance. However, it failed to detect DENV-1 variants in Cambodia due to a point mutation. We describe an improvement of the method that allows the detection of additional DENV-1 strains, including potential variants.
摘要
兰乔蒂及其同事（R.S.兰乔蒂、C.H.卡利舍尔、D.J.古布勒、G.J.张和A.万斯 - 沃恩达姆，《临床微生物学杂志》30:545 - 551，1992年）所描述的登革病毒分子分型方法在全球范围内用于诊断和监测。然而，由于一个点突变，该方法未能在柬埔寨检测到登革病毒1型（DENV - 1）变体。我们描述了该方法的一种改进，它能够检测到更多的DENV - 1毒株，包括潜在变体。
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