Adah Mohammed I, Nagashima Shigeo, Wakuda Mitsutaka, Taniguchi Koki
Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192, Japan.
J Clin Microbiol. 2003 Aug;41(8):3945-50. doi: 10.1128/JCM.41.8.3945-3950.2003.
A bovine rotavirus, NGRBg8, isolated from the feces of a calf with diarrhea in Nigeria was characterized by reverse transcription-PCR, nucleotide sequence analysis, and Northern blot hybridization. The nucleotide sequence of the VP7 gene of the strain was most closely related to that of a Nigerian human G8-serotype strain, HMG035 (99.9%). The NSP1 gene of strain NGRBg8 is highly related (99.4%) to that of a Thai G8 bovine strain, A5-10. Northern blot hybridization revealed a high overall genomic relatedness of bovine strain NGRBg8 with human strain HMG035; all 11 RNA segments hybridized to each other. Thus, the results show the close relationship between G8 bovine and human rotaviruses in Nigeria.
从尼日利亚一头腹泻小牛粪便中分离出的牛轮状病毒NGRBg8,通过逆转录聚合酶链反应、核苷酸序列分析和Northern印迹杂交进行了特性鉴定。该毒株VP7基因的核苷酸序列与尼日利亚人G8血清型毒株HMG035的序列关系最为密切(99.9%)。NGRBg8毒株的NSP1基因与泰国G8牛毒株A5 - 10的基因高度相关(99.4%)。Northern印迹杂交显示牛毒株NGRBg8与人类毒株HMG035在基因组上总体相关性较高;所有11个RNA片段相互杂交。因此,结果表明尼日利亚G8牛轮状病毒和人轮状病毒之间关系密切。