Dupin Nicolas, Bijaoui Gérard, Schwarzinger Michaël, Ernault Pauline, Gerhardt Philippe, Jdid Randa, Hilab Salim, Pantoja Christina, Buffet Marc, Escande Jean-Paul, Costa Jean-Marc
Service de Dermato-Vénéréologie, Hôpital Cochin, Université René Descartes, Paris, France.
Clin Infect Dis. 2003 Aug 15;37(4):602-5. doi: 10.1086/376990. Epub 2003 Jul 30.
Detection and quantification of Mycoplasma genitalium were evaluated in 83 patients with urethritis (group 1), 60 patients with urethral symptoms but no urethritis (group 2), and 50 asymptomatic men (group 3). Quantification of M. genitalium was carried out using real-time polymerase chain reaction (PCR) analysis of first-pass urine samples. The rate of detection of M. genitalium was significantly higher in group 1 than in groups 2 and 3 (P<.0001). The mean observed concentration of M. genitalium was 1.2x10(4) equivalent genomes/mL of urine (range, 50 to 8x10(4) equivalent genomes/mL). Analysis of M. genitalium load in serial urine samples collected before and after the administration of antibacterial treatment showed an association between clinical and microbiological responses, with a shift to negative PCR results in symptom-free patients. Our results illustrate the usefulness of monitoring the M. genitalium load in evaluating the susceptibility of M. genitalium to antibacterial treatment.
对83例尿道炎患者（第1组）、60例有尿道症状但无尿道炎的患者（第2组）和50例无症状男性（第3组）进行了生殖支原体的检测和定量分析。采用实时聚合酶链反应（PCR）分析首次排尿尿液样本对生殖支原体进行定量。第1组生殖支原体的检出率显著高于第2组和第3组（P<0.0001）。生殖支原体的平均检测浓度为1.2×10⁴等效基因组/mL尿液（范围为50至8×10⁴等效基因组/mL）。对抗菌治疗前后采集的系列尿液样本中的生殖支原体载量分析显示，临床反应与微生物学反应之间存在关联，无症状患者的PCR结果转为阴性。我们的结果说明了监测生殖支原体载量在评估生殖支原体对抗菌治疗敏感性方面的有用性。
