Busolo F, Camposampiero D, Bordignon G, Bertollo G
Institute of Microbiology of Padua University, Faculty of Medicine, Italy.
New Microbiol. 1997 Oct;20(4):325-32.
The use of PCR assays as a fast and reliable method is constantly improving and easing microbiological diagnosis. We used a polymerase chain reaction (PCR) assay designed to detect Mycoplasma genitalium and Chlamydia trachomatis in urethral swab samples of 56 males with urethritis and 44 asymptomatic patients as a control group. The PCR assay provides an amplification of target sequence within MgPa (M. genitalium protein attachment) gene. Results indicated that M. genitalium was present in 6 (10.7%) patients with urethritis and none in the control group. Eleven of 56 (17.8%) patients were positive for Chlamydia trachomatis when tested by an outer membrane protein primer-based PCR. The amplified DNA fragments were homogeneous as shown by restriction enzyme analysis and found to be consistent with the published sequences. The PCR assay employed was as reliable as the cultural method in detecting C. trachomatis in the urethral swabs of patients with urethritis (100% of sensitivity when compared with the cultural method) and it has been revealed as an essential method for detection of M. genitalium.
聚合酶链反应（PCR）检测作为一种快速可靠的方法，其应用正在不断改进并简化微生物学诊断。我们采用了一种聚合酶链反应（PCR）检测方法，该方法旨在检测56例尿道炎男性患者尿道拭子样本中的生殖支原体和沙眼衣原体，并以44例无症状患者作为对照组。PCR检测可扩增MgPa（生殖支原体蛋白附着）基因内的目标序列。结果显示，6例（10.7%）尿道炎患者存在生殖支原体，而对照组中未发现。采用基于外膜蛋白引物的PCR检测时，56例患者中有11例（17.8%）沙眼衣原体呈阳性。经限制性酶切分析表明，扩增的DNA片段具有同源性，且与已发表序列一致。所采用的PCR检测在检测尿道炎患者尿道拭子中的沙眼衣原体方面与培养法一样可靠（与培养法相比，灵敏度为100%），并且已被证明是检测生殖支原体的重要方法。
