Kuo Wen-Chuan, Chou Chien, Wu Hsieh-Ting
Institute of Radiological Science and Department of Medical Radiation Technology, National Yang-Ming University, Pei-Tou, Taipei 112, Taiwan.
Opt Lett. 2003 Aug 1;28(15):1329-31. doi: 10.1364/ol.28.001329.
A novel optical heterodyne surface-plasmon resonance (SPR) biosensor with a Zeeman laser is proposed. Two surface plasma waves are excited by two correlated p-polarized waves in a SPR device of the Kretschmann configuration. Two reflected p waves are optically heterodyned such that the magnitude of the heterodyned signal is proportional to the multiplication of two attenuated reflected p waves. Then the detection sensitivity and the dynamic range based on this amplitude-sensitive method are enhanced. In the experiment, the kinetics between mouse immunoglobulin G (IgG) and rabbit antimouse IgG is obtained from sensograms of various concentrations of antimouse IgG. A detection sensitivity of 0.2 nM was achieved. In addition, a concentration of 5 ng/ml of protein G interacting with mouse IgG was measured successfully.
