Pawlikowska-Pawlega Bozena, Gruszecki Wieslaw I, Misiak Lucjan E, Gawron Antoni
Department of Comparative Anatomy and Anthropology, Maria Curie-Sklodowska University, Ul. Akademicka 19, 20-033 Lublin, Poland.
Biochem Pharmacol. 2003 Aug 15;66(4):605-12. doi: 10.1016/s0006-2952(03)00344-7.
Quercetin is a naturally occurring flavonoid that exerts multiple pharmacological effects. In our previous study, we showed that quercetin greatly affects the lipid membrane. In this report, a study of quercetin on human erythrocyte membrane has been performed to determine the influence of this flavonoid on the fluidity and the conformational changes of membrane proteins. An additional aim of the study was to find how quercetin presence affects the resistance of membrane to haemolytic agents. The results showed that incorporation of quercetin into the erythrocyte membranes caused the changes of the partition coefficient of the Tempo spin label between the water and polar head group phases. In the studies, the W/S ratio has been used as a monitor of changes in protein conformation and in the environment within the membrane. It was observed that quercetin caused an increase in protein-protein interactions in human erythrocyte membranes. Haemolytic action of quercetin in the dark was also investigated. This compound showed protective effect against hypotonic haemolysis. However, in the heat-induced haemolysis quercetin caused acceleration of haemolysis. Dark reaction of erythrocyte with quercetin resulted in a shrinkage of the cells and alteration of their shapes. From the results we have concluded that modification of erythrocyte membrane by quercetin proceeds via reaction with membrane lipids and proteins.
槲皮素是一种具有多种药理作用的天然黄酮类化合物。在我们之前的研究中，我们表明槲皮素对脂质膜有很大影响。在本报告中，我们对槲皮素作用于人体红细胞膜进行了研究，以确定这种黄酮类化合物对膜流动性和膜蛋白构象变化的影响。该研究的另一个目的是了解槲皮素的存在如何影响膜对溶血剂的抗性。结果表明，槲皮素掺入红细胞膜会导致Tempo自旋标记物在水相和极性头部基团相之间的分配系数发生变化。在研究中，W/S比值被用作监测膜内蛋白质构象和环境变化的指标。据观察，槲皮素会导致人体红细胞膜中蛋白质-蛋白质相互作用增加。我们还研究了槲皮素在黑暗中的溶血作用。该化合物对低渗溶血具有保护作用。然而，在热诱导溶血中，槲皮素会加速溶血。红细胞与槲皮素的黑暗反应导致细胞收缩并改变其形状。从结果中我们得出结论，槲皮素对红细胞膜的修饰是通过与膜脂质和蛋白质反应进行的。
