Lavertu M, Xia Z, Serreqi A N, Berrada M, Rodrigues A, Wang D, Buschmann M D, Gupta Ajay
Department of Chemical Engineering, Institute of Biomedical and Chemical Engineering, Ecole Polytechnique of Montréal, PO Box 6079, Station Center-Ville, Québec, Montréal, Canada.
J Pharm Biomed Anal. 2003 Aug 21;32(6):1149-58. doi: 10.1016/s0731-7085(03)00155-9.
A method for the determination of the degree of deacetylation (DDA) of chitosan by 1H NMR spectroscopy has been formally validated. Chitosans with DDA ranging from 48 to 100% have been used for the validation. The method is found to be simple, rapid and more precise than other known techniques like IR or titration for %DDA measurements. The precision, ruggedness, robustness, specificity, stability and accuracy of the technique are discussed in this paper.
一种通过¹H NMR光谱法测定壳聚糖脱乙酰度（DDA）的方法已得到正式验证。验证过程中使用了脱乙酰度范围为48%至100%的壳聚糖。结果发现，该方法相较于红外光谱法或滴定法等其他已知的%DDA测量技术，具有简单、快速且更精确的特点。本文讨论了该技术的精密度、耐用性、稳健性、特异性、稳定性和准确性。
