Baxter A, Dillon M, Taylor K D, Roberts G A
Department of Food Studies, Humberside Polytechnic, Grimsby, UK.
Int J Biol Macromol. 1992 Jun;14(3):166-9. doi: 10.1016/s0141-8130(05)80007-8.
Three published i.r. absorption band ratios for determining the % N-acetylation of chitosan have been used to follow the rate of alkaline deacetylation of chitin. Only one, the A1655/A3450 ratio, gives the expected rate of deacetylation curve. Consideration of the various i.r. ratios has led to a new relationship, % N-acetylation = (A1655/A3450) x 115, where the absorbance of the amide I band at approximately 1655 cm-1 is determined using the baseline proposed previously. The values obtained using this proposed relationship agree closely with those obtained from dye adsorption measurements for chitosans having % N-acetylation values in the range 0-55%.
已使用三种已发表的用于测定壳聚糖N - 乙酰化百分比的红外吸收带比率来跟踪几丁质的碱性脱乙酰化速率。只有一个比率，即A1655/A3450比率，给出了预期的脱乙酰化曲线速率。对各种红外比率的研究得出了一个新的关系式，N - 乙酰化百分比 = (A1655/A3450)×115，其中在约1655 cm-1处酰胺I带的吸光度是使用先前提出的基线来确定的。使用这个提议的关系式获得的值与通过染料吸附测量法对N - 乙酰化值在0 - 55%范围内的壳聚糖所获得的值非常吻合。
