Tritean Naomi, Dimitriu Luminița, Dima Ștefan-Ovidiu, Ghiurea Marius, Trică Bogdan, Nicolae Cristian-Andi, Moraru Ionuț, Nicolescu Alina, Cimpean Anisoara, Oancea Florin, Constantinescu-Aruxandei Diana
Bioresource and Polymer Department, National Institute for Research & Development in Chemistry and Petrochemistry-ICECHIM, Splaiul Independenței nr. 202, Sector 6, 060021 Bucharest, Romania.
Faculty of Biology, University of Bucharest, Spl. Independentei nr. 91-95, Sector 5, 50095 Bucharest, Romania.
J Funct Biomater. 2024 Jul 22;15(7):202. doi: 10.3390/jfb15070202.
Selenium nanoparticles (SeNPs) have specific properties that result from their biosynthesis particularities. Chitosan can prevent pathogenic biofilm development. A wide palette of bacterial nanocellulose (BNC) biological and physical-chemical properties are known. The aim of this study was to develop a hydrogel formulation (SeBNCSFa) based on ferulic acid-grafted chitosan and bacterial nanocellulose (BNC) enriched with SeNPs from Kombucha fermentation (SeNPsK), which could be used as an adjuvant for oral implant integration and other applications. The grafted chitosan and SeBNCSFa were characterized by biochemical and physical-chemical methods. The cell viability and proliferation of HGF-1 gingival fibroblasts were investigated, as well as their in vitro antioxidant activity. The inflammatory response was determined by enzyme-linked immunosorbent assay (ELISA) of the proinflammatory mediators (IL-6, TNF-α, and IL-1β) in cell culture medium. Likewise, the amount of nitric oxide released was measured by the Griess reaction. The antimicrobial activity was also investigated. The grafting degree with ferulic acid was approximately 1.780 ± 0.07% of the total chitosan monomeric units, assuming single-site grafting per monomer. Fourier-transform infrared spectroscopy evidenced a convolution of BNC and grafted chitosan spectra, and X-ray diffraction analysis highlighted an amorphous rearrangement of the diffraction patterns, suggesting multiple interactions. The hydrogel showed a high degree of cytocompatibility, and enhanced antioxidant, anti-inflammatory, and antimicrobial potentials.
硒纳米颗粒（SeNPs）因其生物合成特性而具有特定性质。壳聚糖可防止致病性生物膜形成。人们已知细菌纳米纤维素（BNC）具有广泛的生物学和物理化学性质。本研究的目的是开发一种基于阿魏酸接枝壳聚糖和富含来自康普茶发酵的硒纳米颗粒（SeNPsK）的细菌纳米纤维素（BNC）的水凝胶制剂（SeBNCSFa），其可作为口腔种植体整合及其他应用的佐剂。对接枝壳聚糖和SeBNCSFa进行了生化和物理化学方法表征。研究了HGF-1牙龈成纤维细胞的细胞活力和增殖情况，以及它们的体外抗氧化活性。通过对细胞培养基中促炎介质（IL-6、TNF-α和IL-1β）进行酶联免疫吸附测定（ELISA）来确定炎症反应。同样，通过格里斯反应测量释放的一氧化氮量。还研究了抗菌活性。假设每个单体进行单位点接枝，阿魏酸的接枝度约为壳聚糖总单体单元的1.780±0.07%。傅里叶变换红外光谱证明了BNC和接枝壳聚糖光谱的卷积，X射线衍射分析突出了衍射图谱的无定形重排，表明存在多种相互作用。该水凝胶表现出高度的细胞相容性，并具有增强的抗氧化、抗炎和抗菌潜力。
