一氧化氮代谢产物在原发性纤毛运动障碍患者的呼出气冷凝物中并未减少。

Nitric oxide metabolites are not reduced in exhaled breath condensate of patients with primary ciliary dyskinesia.

作者信息

Csoma Zsuzsanna, Bush Andrew, Wilson Nicola M, Donnelly Louise, Balint Beatrix, Barnes Peter J, Kharitonov Sergei A

机构信息

Department of Thoracic Medicine, Imperial College School of Medicine, National Heart and Lung Institute, London, UK.

出版信息

Chest. 2003 Aug;124(2):633-8. doi: 10.1378/chest.124.2.633.

DOI:10.1378/chest.124.2.633

PMID:12907553

Abstract

STUDY OBJECTIVES

To investigate whether nitric oxide (NO) metabolites would be reduced in children affected by primary ciliary dyskinesia (PCD).

DESIGN

Single-center observational study.

PATIENTS

Fifteen children with PCD (seven boys; mean [+/- SEM] age, 10.3 +/- 0.7 years; mean FEV(1), 73 +/- 2.1% predicted) were recruited along with 14 healthy age-matched subjects (seven boys; mean age, 11.5 +/- 0.4 years; mean FEV(1), 103 +/- 5% predicted).

INTERVENTIONS

We assessed the levels of nitrite (NO(2)(-)), NO(2)(-)/NO(3)(-) (NO(2)(-)/NO(3)(-)), and S-nitrosothiol in exhaled breath condensate, exhaled NO, and nasal NO from children with PCD compared to those in healthy children.

MEASUREMENTS AND RESULTS

The mean exhaled and nasal NO levels were markedly decreased in children with PCD compared to those without PCD (3.2 +/- 0.2 vs 8.5 +/- 0.9 parts per billion [ppb], respectively [p < 0.0001]; 59.6 +/- 12.2 vs 505.5 +/- 66.8 ppb, respectively [p < 0.001]). Despite the lower levels of exhaled NO in children with PCD, no differences were found in the mean levels of NO(2)(-) (2.9 +/- 0.4 vs 3.5 +/- 0.3 microM, respectively), NO(2)(-)/NO(3)(-) (35.2 +/- 5.0 vs 34.3 +/- 4.5 microM, respectively), or S-nitrosothiol (1.0 +/- 0.2 vs 0.6 +/- 0.1 microM, respectively) between children with PCD and healthy subjects.

CONCLUSION

These findings suggest that NO synthase activity may not be decreased as much as might be expected on the basis of low exhaled and nasal NO levels.

摘要

研究目的

调查原发性纤毛运动障碍（PCD）患儿的一氧化氮（NO）代谢产物是否会减少。

设计

单中心观察性研究。

患者

招募了15名PCD患儿（7名男孩；平均[±标准误]年龄为10.3±0.7岁；平均第一秒用力呼气容积[FEV(1)]为预测值的73±2.1%）以及14名年龄匹配的健康受试者（7名男孩；平均年龄为11.5±0.4岁；平均FEV(1)为预测值的103±5%）。

干预措施

我们评估了PCD患儿与健康儿童相比，呼出气冷凝物、呼出NO和鼻腔NO中的亚硝酸盐（NO₂⁻）、NO₂⁻/NO₃⁻（NO₂⁻/NO₃⁻）以及S-亚硝基硫醇水平。

测量与结果

与无PCD的儿童相比，PCD患儿的平均呼出和鼻腔NO水平显著降低（分别为3.2±0.2 vs 8.5±0.9十亿分之一[ppb]，[p < 0.0001]；分别为59.6±12.2 vs 505.5±66.8 ppb，[p < 0.001]）。尽管PCD患儿呼出NO水平较低，但PCD患儿与健康受试者之间在NO₂⁻平均水平（分别为2.9±0.4 vs 3.5±0.3微摩尔/升）、NO₂⁻/NO₃⁻平均水平（分别为35.2±5.0 vs 34.3±4.5微摩尔/升）或S-亚硝基硫醇平均水平（分别为1.0±0.2 vs 0.6±0.1微摩尔/升）方面未发现差异。