Rahimi Kamal, Goli Rasoul, Faraji Navid, Pourheidar Bagher, Nabavi Somaye, Pourheidar Maryam, Babamiri Behnam
Department of Nursing, School of Nursing and Midwifery, Urmia University of Medical Sciences, Urmia, Islamic Republic of Iran.
Department of Medical-Surgical Nursing, School of Nursing and Midwifery, Urmia University of Medical Sciences, Urmia, Islamic Republic of Iran.
Toxicol Rep. 2023 Sep 5;11:241-248. doi: 10.1016/j.toxrep.2023.08.005. eCollection 2023 Dec.
Diabetes causes testicular damage due to oxidative stress. Nowadays, the use of vitamins and antioxidants is one of the common methods to treat this disease. Therefore, the aim of this study is to investigate the effect of single and combined administration of these two substances on the reproductive system of male diabetic rats.
In this study, 48 adult male Wistar rats weighing 250-270 grammes were divided into 6 groups: Control group, diabetic group, vehicle group, vitamin E, curcumin group, and vitamin E and curcumin group. The control group was the healthy group, and in the other groups, the rats were made diabetic by streptozotocin (60 mg/kg/ip). The vehicle group received 1 ml of olive oil, the vitamin E group (100 mg/kg/ip) received Vit.E, and the curcumin group (50 mg/kg/ip) received Cu. The group of rats received vitamin E and curcumin. At the end of the sixth week after treatment, blood was taken from the rats and biochemical analysis was performed to check the amount of malondialdehyde (MDA), LH hormones and serum testosterone, then the rats were killed and their testes and epididymides were removed. The weight of the testes and sperm parameters, the maturity of sperm nuclei and the integrity of their DNA were checked. The number of spermatogenic cells was determined by histological examination.
This study showed that diabetes caused a decrease in testicular weight, sperm count, motility, and viability, an increased percentage of sperm with immature nuclei, and an increased percentage of sperm with denatured DNA. In addition, diabetes decreased the average number of matogenic sperm, and biochemical results showed that diabetes increased the level of MDA and decreased the level of the hormones LH and testosterone. Treatment with vitamin E, curcumin and their combination improved all these parameters, and this improvement was significant in the Toam group.
Combined administration of vitamin E and curcumin in diabetic rats significantly improves sperm parameters, matogenic sperm count, and improves MDA levels, LH, and serum testosterone compared with separate treatment.
糖尿病因氧化应激导致睾丸损伤。如今，使用维生素和抗氧化剂是治疗该疾病的常用方法之一。因此，本研究的目的是探讨这两种物质单独及联合给药对雄性糖尿病大鼠生殖系统的影响。
在本研究中，将48只体重250 - 270克的成年雄性Wistar大鼠分为6组：对照组、糖尿病组、赋形剂组、维生素E组、姜黄素组以及维生素E和姜黄素组。对照组为健康组，其他组大鼠通过链脲佐菌素（60毫克/千克/腹腔注射）诱导成糖尿病。赋形剂组接受1毫升橄榄油，维生素E组（100毫克/千克/腹腔注射）接受维生素E，姜黄素组（50毫克/千克/腹腔注射）接受姜黄素。大鼠组接受维生素E和姜黄素。治疗六周结束后，从大鼠身上采血并进行生化分析，以检测丙二醛（MDA）、促黄体生成素（LH）激素和血清睾酮的含量，然后处死大鼠并取出其睾丸和附睾。检查睾丸重量、精子参数、精子核成熟度及其DNA完整性。通过组织学检查确定生精细胞数量。
本研究表明，糖尿病导致睾丸重量、精子数量、活力和存活率下降，未成熟核精子百分比增加，变性DNA精子百分比增加。此外，糖尿病降低了生精精子的平均数量，生化结果显示糖尿病增加了MDA水平，降低了LH和睾酮激素水平。维生素E、姜黄素及其组合治疗改善了所有这些参数，且在联合组中这种改善具有显著性。
与单独治疗相比，糖尿病大鼠联合使用维生素E和姜黄素可显著改善精子参数、生精精子数量，并改善MDA水平、LH和血清睾酮水平。
