Mostyn A, Wilson V, Dandrea J, Yakubu D P, Budge H, Alves-Guerra M C, Pecqueur C, Miroux B, Symonds M E, Stephenson T
Academic Division of Child Health, School of Human Development, Queen's Medical Centre, University Hospital, Nottingham NG7 2UH, UK.
Br J Nutr. 2003 Aug;90(2):323-8. doi: 10.1079/bjn2003912.
The present study examined the ontogeny of mitochondrial protein abundance in adipose tissue and lungs over the first month of life in the sheep and the extent to which this may be altered by maternal undernutrition during the final month of gestation. The ontogeny of uncoupling protein (UCP), voltage-dependent anion channel (VDAC) and cytochrome c abundance were determined in adipose tissue and lungs sampled from near-term fetuses and young sheep aged 4 h, 1, 7 and 30 d. In adipose tissue, the abundance of UCP1, VDAC and cytochrome c all peaked at 1 d of age and then decreased by 30 d of age, at which stage the brown adipose tissue-specific UCP1 was no longer detectable but UCP2 was clearly abundant. For the lungs, however, UCP2 and VDAC abundance both peaked 7 d after birth and then decreased by 30 d of age. During postnatal development, therefore, a marked change in mitochondrial protein abundance occurs within both adipose tissue and lungs. Maternal nutrient restriction had no effect on lamb growth or tissue weights at 30 d of age but was associated with increased abundance of UCP2 and VDAC but not cytochrome c in both adipose tissue and lungs. These mitochondrial adaptations within both adipose tissue and the lungs of offspring born to previously nutrient-restricted mothers may compromise adipose tissue and lung function during periods of environmental stress.
本研究考察了绵羊出生后第一个月内脂肪组织和肺中线粒体蛋白丰度的个体发生情况，以及妊娠最后一个月母体营养不足对此可能产生的影响程度。在取自近期胎儿以及出生4小时、1天、7天和30天的幼羊的脂肪组织和肺中，测定了解偶联蛋白（UCP）、电压依赖性阴离子通道（VDAC）和细胞色素c的丰度。在脂肪组织中，UCP1、VDAC和细胞色素c的丰度均在1日龄时达到峰值，然后在30日龄时下降，此时棕色脂肪组织特异性UCP1不再可检测到，但UCP2明显丰富。然而，对于肺来说，UCP2和VDAC的丰度在出生后7天均达到峰值，然后在30日龄时下降。因此，在出生后的发育过程中，脂肪组织和肺中线粒体蛋白丰度均发生显著变化。母体营养限制对30日龄羔羊的生长或组织重量没有影响，但与脂肪组织和肺中UCP2和VDAC丰度增加有关，而与细胞色素c无关。先前营养受限的母羊所生后代的脂肪组织和肺中的这些线粒体适应性变化，可能会在环境应激期间损害脂肪组织和肺功能。
