Smolinski Alexa T, Pestka James J
Department of Food Science and Human Nutrition, Michigan State University, East Lansing, MI 48824-1224, USA.
Food Chem Toxicol. 2003 Oct;41(10):1381-90. doi: 10.1016/s0278-6915(03)00146-7.
Dietary supplements are not subject to the same pre-market approval as conventional drugs, thus the true efficacy and, in cases, safety of these products is not known. The objective of this study was to evaluate the potential anti-inflammatory properties of three herbal constituents, apigenin (chamomile), ginsenoside Rb(1) (ginseng) and parthenolide (feverfew) on lipopolysaccaharide (LPS)-induced proinflammatory cytokine production, and to determine if effects in cell culture could predict results in an intact animal model. Murine macrophage cells and mice were treated with the stimulant LPS and herbal constituents, and resultant culture supernatant and serum, respectively, were evaluated for interleukin (IL)-6 and tumor necrosis factor (TNF)-alpha by ELISA. All three constituents inhibited LPS-induced IL-6 and/or TNF-alpha production in culture. Inhibition of these two cytokines was observed in mice, but did not display the same patterns of inhibition as cell culture data. The results suggest that all three constituents possessed anti-inflammatory properties, but that cell culture data can only be used to approximate potential effects in animals, and must be confirmed using appropriate animal models.
膳食补充剂不像传统药物那样需要经过相同的上市前审批，因此这些产品的真正功效以及在某些情况下的安全性尚不清楚。本研究的目的是评估三种草药成分，即芹菜素（洋甘菊）、人参皂苷Rb(1)（人参）和小白菊内酯（小白菊）对脂多糖（LPS）诱导的促炎细胞因子产生的潜在抗炎特性，并确定细胞培养中的作用是否可以预测完整动物模型中的结果。用刺激物LPS和草药成分处理小鼠巨噬细胞和小鼠，然后分别通过ELISA评估所得的培养上清液和血清中的白细胞介素（IL）-6和肿瘤坏死因子（TNF）-α。所有三种成分均抑制培养物中LPS诱导的IL-6和/或TNF-α产生。在小鼠中观察到这两种细胞因子受到抑制，但抑制模式与细胞培养数据不同。结果表明，所有三种成分都具有抗炎特性，但细胞培养数据只能用于大致估计在动物中的潜在作用，必须使用适当的动物模型进行验证。
