Daugé V, Kalivas P W, Duffy T, Roques B P
Département de Pharmacochimie Moléculaire et Structurale, U266 INSERM, URA D1500 CNRS, Faculté de Pharmacie, Paris, France.
Brain Res. 1992 Dec 25;599(2):209-14. doi: 10.1016/0006-8993(92)90393-n.
The mixed inhibitor of enkephalin catabolism, kelatorphan, was microinjected into the ventral tegmental area (VTA) of rats to determine if endogenous enkephalins can modulate dopamine transmission in the mesoaccumbens projection. The concentration of extracellular dopamine content in the nucleus accumbens was monitored using in vivo microdialysis simultaneously with measuring motor behavior. Kelatorphan microinjection into the VTA produced a dose-related increase in motor activity and extracellular dopamine in the nucleus accumbens. While the change in extracellular dopamine was modest as compared to exogenous stimulation by a mu agonist such as DAMGO, there was a marked increase in the extracellular content of dopamine and serotonin metabolites. This suggests that mesoaccumbens dopamine transmission is under tonic control of endogenous enkephalins at the ventral tegmental area level.
将脑啡肽分解代谢的混合抑制剂凯拉托芬微量注射到大鼠腹侧被盖区（VTA），以确定内源性脑啡肽是否能调节中伏隔核投射中的多巴胺传递。在测量运动行为的同时，使用体内微透析监测伏隔核中细胞外多巴胺含量的浓度。向VTA微量注射凯拉托芬会使运动活动以及伏隔核中的细胞外多巴胺呈剂量相关增加。虽然与μ激动剂如DAMGO的外源性刺激相比，细胞外多巴胺的变化不大，但多巴胺和5-羟色胺代谢产物的细胞外含量有显著增加。这表明中伏隔核多巴胺传递在腹侧被盖区水平受到内源性脑啡肽的紧张性控制。
