Lee J-J, Nam C-E, Cho S-H, Park K-S, Chung I-J, Kim H-J
Department of Internal Medicine, Chonnam National University Medical School, 8 Hakdong, Dongku, 501-757, Gwangju, Korea.
Genome Research Center for Hematopoietic Diseases, Chonnam National University Hospital, Gwangju, Korea.
Ann Hematol. 2003 Aug;82(8):492-495. doi: 10.1007/s00277-003-0691-4. Epub 2003 Jun 21.
We investigated telomere length changes in patients with non-Hodgkin's lymphoma (NHL) receiving conventional-dose chemotherapy. Using Southern blot analysis, telomere length was measured in peripheral blood mononuclear cells from five NHL patients at diagnosis, 15 NHL patients after chemotherapy, and 39 healthy controls. Compared with age-matched putative normal controls, telomeres were significantly shorter in NHL patients at diagnosis. Mean telomere length was shorter after chemotherapy than before chemotherapy and was shorter after chemotherapy than in age-matched putative healthy controls. There was no correlation between the extent of telomere shortening and time elapsed after chemotherapy. These findings suggest that in NHL patients hematopoietic stem cells lose telomere length during the recovery period from bone marrow suppression after conventional-dose chemotherapy.
我们研究了接受常规剂量化疗的非霍奇金淋巴瘤（NHL）患者的端粒长度变化。采用Southern印迹分析，测量了5例初诊NHL患者、15例化疗后NHL患者及39名健康对照外周血单个核细胞的端粒长度。与年龄匹配的假定正常对照相比，初诊NHL患者的端粒明显缩短。化疗后的平均端粒长度比化疗前短，且化疗后比年龄匹配的假定健康对照短。端粒缩短程度与化疗后经过的时间之间无相关性。这些发现提示，在NHL患者中，造血干细胞在常规剂量化疗后骨髓抑制恢复期会丢失端粒长度。
