Kwon Chong-Suk, Sohn Ho Yong, Kim Sung Hee, Kim Ji Hyun, Son Kun Ho, Lee Jeong Soon, Lim Jin Kyu, Kim Jong-Sang
Department of Food Science and Nutrition, Andong National University, S. Korea.
Biosci Biotechnol Biochem. 2003 Jul;67(7):1451-6. doi: 10.1271/bbb.67.1451.
In the process of screening for pancreatic lipase inhibitors, which could be used as an anti-obesity measure, the methanol extract of Dioscorea nipponica Makino powder (DP) appeared to have potent inhibitory activity against porcine pancreatic lipase with an IC50 value of 5-10 microg/ml, where the enzyme activity was assayed by using 4-methylumbelliferyl oleate as a substrate. Further purification of active components present in the herb generated dioscin that belongs to the saponin family. Dioscin and its aglycone, diosgenin, both suppressed the time-dependent increase of blood triacylglycerol level when orally injected with corn oil to mice, suggesting their inhibitory potential against fat absorption. Sprague-Dawley rats fed on a high-fat diet containing 5% Dioscorea nipponica Makino and 40% beef tallow gained significantly less body weight and adipose tissue than control animals fed on a high-fat diet alone during an 8-week experimental period (P<0.05).
在筛选可作为抗肥胖措施的胰腺脂肪酶抑制剂的过程中，穿山龙粉末（DP）的甲醇提取物对猪胰腺脂肪酶表现出强大的抑制活性，IC50值为5 - 10微克/毫升，其中酶活性是通过使用4 - 甲基伞形酮油酸酯作为底物来测定的。对该草药中存在的活性成分进行进一步纯化得到了属于皂苷家族的薯蓣皂苷。当给小鼠口服玉米油时，薯蓣皂苷及其苷元薯蓣皂苷元均抑制了血液中三酰甘油水平随时间的升高，表明它们对脂肪吸收具有抑制潜力。在为期8周的实验期内，喂食含5%穿山龙和40%牛脂的高脂饮食的斯普拉格 - 道利大鼠比仅喂食高脂饮食的对照动物体重和脂肪组织增加明显更少（P<0.05）。
