Barros Nieves, Feijóo Sergio
Faculty of Physics, Department of Applied Physics, University of Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Biophys Chem. 2003 Jul 1;104(3):561-72. doi: 10.1016/s0301-4622(03)00059-0.
In this work, a method is proposed to quantify the efficiency of carbon utilization by soil microbes. Microcalorimetry was used to compute the heat yield (Y(Q/X)) of six soil samples collected in the Amazon. A combined mass and energy balance is developed to quantify the enthalpy of the glucose oxidation reaction (Delta(r)H(s)) and the biomass yield (Y(X/S)) from the experimental values of Y(Q/X). Results were compared by graphical analysis to establish the kinetics of the glucose oxidation and the microbial growth reactions in terms of energy dissipation. The correlations found suggest that the measured values for Y(Q/X) and Delta(r)H(s) are biomass yield dependent. The main environmental factors affecting the kinetics of the glucose oxidation and the microbial growth reactions in soils are the initial microbial population and the percentage of nitrogen of the samples. The comparative study among the samples showed that the deforestation of the Primary forests in the Amazon to establish arable lands, affected the efficiency of the carbon utilization by soil microorganisms.
在这项工作中，提出了一种量化土壤微生物碳利用效率的方法。微量热法用于计算在亚马逊地区采集的六个土壤样品的热产率（Y(Q/X)）。建立了质量和能量联合平衡，以根据Y(Q/X)的实验值量化葡萄糖氧化反应的焓（Δ(r)H(s)）和生物量产率（Y(X/S)）。通过图形分析比较结果，以确定葡萄糖氧化和微生物生长反应在能量耗散方面的动力学。发现的相关性表明，Y(Q/X)和Δ(r)H(s)的测量值取决于生物量产率。影响土壤中葡萄糖氧化和微生物生长反应动力学的主要环境因素是初始微生物种群和样品中的氮含量。样品之间的比较研究表明，亚马逊地区原始森林的砍伐以建立耕地，影响了土壤微生物的碳利用效率。
