Jiangsu Provincial Key Laboratory of Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Organic Solid Waste Utilization, and National Engineering Research Center for Organic-based Fertilizer, Nanjing Agricultural University, Nanjing, 210095, China.
Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and the Environment, Fujian Agriculture & Forestry University, Fuzhou, 350002, China.
Environ Sci Pollut Res Int. 2017 Oct;24(28):22485-22493. doi: 10.1007/s11356-017-9839-y. Epub 2017 Aug 12.
Volatile organic compounds (VOCs) from soil bacteria are likely to have an important role in the interactions among soil microorganisms. However, their effects on the soil microbial community have not been extensively studied. In this study, the effect of bacterial VOCs generated by growing Bacillus amyloliquefaciens NJN-6 on modified MS medium on soil microbial community was evaluated. B. amyloliquefaciens NJN-6 was able to produce 48 volatile compounds as determined by solid-phase microextraction-GC/MS. MiSeq sequencing data showed that bacterial VOCs could alter the composition of both soil bacterial and soil fungal communities and could decrease the alpha-diversity of the soil microbial community. Taxonomic analysis revealed that bacterial VOCs significantly increased the relative abundance of Proteobacteria, Bacteroidetes, and Firmicutes. Moreover, bacterial VOCs significantly increased the relative abundance of Ascomycota. The qPCR data showed that bacterial VOCs of strain NJN-6 decreased the soil fungal biomass and increased the soil bacterial biomass. Further evaluation of the effect of bacterial VOCs on functional genes revealed that VOCs could reduce the copies of nifH, nirS, and a gene encoding nonribosomal peptide synthase, while increasing the copy number of the ammonium-oxidizing bacteria gene. The effect on gene encoding polyketide synthase was insignificant. Results from this study indicated that bacterial VOCs could influence the soil microbial community as well as functional gene abundance.
土壤细菌产生的挥发性有机化合物（VOCs）可能在土壤微生物相互作用中发挥重要作用。然而，它们对土壤微生物群落的影响尚未得到广泛研究。本研究评估了在改良 MS 培养基中生长的解淀粉芽孢杆菌 NJN-6 产生的细菌 VOC 对土壤微生物群落的影响。通过固相微萃取-GC/MS 测定，NJN-6 能够产生 48 种挥发性化合物。MiSeq 测序数据显示，细菌 VOC 可以改变土壤细菌和土壤真菌群落的组成，并降低土壤微生物群落的α多样性。分类分析表明，细菌 VOC 显著增加了变形菌门、拟杆菌门和厚壁菌门的相对丰度。此外，细菌 VOC 显著增加了子囊菌门的相对丰度。qPCR 数据显示，菌株 NJN-6 的细菌 VOC 降低了土壤真菌生物量，增加了土壤细菌生物量。进一步评估细菌 VOC 对功能基因的影响表明，VOC 可以降低 nifH、nirS 和非核糖体肽合酶基因的拷贝数，同时增加氨氧化细菌基因的拷贝数。对聚酮合酶基因的影响不显著。本研究结果表明，细菌 VOC 可以影响土壤微生物群落及其功能基因丰度。
