Lian Wen-Chian, Hsiao Hung-Chi, Chou Cheng-Chun
Graduate Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan, ROC.
Int J Food Microbiol. 2003 Sep 15;86(3):293-301. doi: 10.1016/s0168-1605(02)00563-9.
Microencapsulated cells of Bifidobacterium longum B6 and Bifidobacterium infantis CCRC 14633 were prepared by spray drying the cell suspension containing the test organism and 10% (w/w) of the carrier material of either gelatin, soluble starch, skim milk or gum arabic. Survival of these microencapsulated and free cells of bifidobacteria in simulated gastric juice (pH 2.0 and 3.0) and bile solution (0.5% and 2.0%) was then examined. B. infantis CCRC 14633 was more susceptible than B. longum B6 to the simulated gastric environment and bile solution tested. Microencapsulated bifidobacteria exhibited a lower population reduction than free cells during exposure to simulated gastric environment and bile solution. This phenomenon was most pronounced when the test organism was exposed to gastric juice at pH 2.0 or 2.0% bile solution. Moreover, it was also observed that the protective effect exerted by encapsulation with spray drying varied with the carriers used and the strains of bifidobacteria.
通过对含有测试菌株以及10%（w/w）明胶、可溶性淀粉、脱脂乳或阿拉伯胶等载体材料的细胞悬液进行喷雾干燥，制备了长双歧杆菌B6和婴儿双歧杆菌CCRC 14633的微囊化细胞。随后检测了这些双歧杆菌微囊化细胞和游离细胞在模拟胃液（pH 2.0和3.0）以及胆汁溶液（0.5%和2.0%）中的存活率。在测试的模拟胃环境和胆汁溶液中，婴儿双歧杆菌CCRC 14633比长双歧杆菌B6更敏感。在暴露于模拟胃环境和胆汁溶液期间，微囊化双歧杆菌的数量减少比游离细胞更低。当测试菌株暴露于pH 2.0的胃液或2.0%的胆汁溶液中时，这种现象最为明显。此外，还观察到喷雾干燥包囊所发挥的保护作用因所用载体和双歧杆菌菌株而异。
