Schubert S, Cuenca S, Fischer D, Heesemann J
Max von Pettenkofer-Institut, 80336 Munich, Germany.
J Infect Dis. 2000 Oct;182(4):1268-71. doi: 10.1086/315831. Epub 2000 Sep 5.
Production of the siderophore yersiniabactin is associated with virulence in Yersinia species. The genes for biosynthesis and uptake of yersiniabactin are located on a high-pathogenicity island (HPI). The distribution and functioning of the Yersinia HPI were assessed in different Enterobacteriaceae strains isolated from blood cultures and urine samples. In total, 550 clinical isolates from 10 Enterobacteriaceae species were investigated by polymerase chain reaction and DNA hybridization. The Yersinia HPI was most prevalent in Escherichia coli (overall prevalence, 72.3%) and, to a lesser extent, in Klebsiella oxytoca (58.3%), Citrobacter species (25%), Klebsiella pneumonia (17.7%), and Enterobacter species (12.2%). The production of the siderophore yersiniabactin was also demonstrated in these HPI-positive strains by use of a reporter gene bioassay. These findings indicate that the HPI of Yersinia is distributed and functions in clinical specimens of different Enterobacteriaceae species.
铁载体耶尔森菌素的产生与耶尔森菌属的毒力相关。耶尔森菌素生物合成和摄取的基因位于一个高致病性岛（HPI）上。对从血培养物和尿液样本中分离出的不同肠杆菌科菌株评估耶尔森菌HPI的分布和功能。通过聚合酶链反应和DNA杂交对来自10种肠杆菌科菌种的550株临床分离株进行了研究。耶尔森菌HPI在大肠杆菌中最为普遍（总体患病率为72.3%），在较小程度上也存在于产酸克雷伯菌（58.3%）、柠檬酸杆菌属（25%）、肺炎克雷伯菌（17.7%）和肠杆菌属（12.2%）中。通过使用报告基因生物测定法，在这些HPI阳性菌株中也证实了铁载体耶尔森菌素的产生。这些发现表明，耶尔森菌的HPI在不同肠杆菌科菌种的临床标本中分布并发挥作用。
