Mokracka Joanna, Koczura Ryszard, Kaznowski Adam
Department of Microbiology, Institute of Experimental Biology, Adam Mickiewicz University, ul. Fredry 10, 61-701 Poznań, Poland.
FEMS Immunol Med Microbiol. 2004 Jan 15;40(1):51-5. doi: 10.1016/S0928-8244(03)00276-1.
We analyzed the ability of extraintestinal strains of Enterobacter spp. and Citrobacter spp. to employ different siderophore-mediated strategies of iron acquisition. All strains produced iron-chelating compounds. Cross-feeding assays indicated that most isolates of both Enterobacter spp. and Citrobacter spp. excreted catecholate siderophore enterobactin, less produced aerobactin, and single strains excreted hydroxamates different from aerobactin. Besides, we analyzed if the strains had the ability to produce the siderophore yersiniabactin coded by the Yersinia high-pathogenicity island (HPI). The presence of HPI genes was observed in single isolates of three species: E. cloaceae, E. aerogenes and C. koseri. A detailed polymerase chain reaction analysis revealed differences in the genetic organization of the HPIs; however, in a cross-feeding test we proved that yersiniabactin was produced and the island was functional.
我们分析了肠杆菌属和柠檬酸杆菌属的肠外菌株采用不同铁载体介导的铁获取策略的能力。所有菌株均产生铁螯合化合物。交叉喂养试验表明，肠杆菌属和柠檬酸杆菌属的大多数分离株分泌儿茶酚型铁载体肠杆菌素，较少产生气杆菌素，单个菌株分泌不同于气杆菌素的异羟肟酸。此外，我们分析了这些菌株是否有能力产生由耶尔森菌高致病性岛（HPI）编码的铁载体耶尔森菌素。在三种菌的单个分离株中观察到HPI基因的存在：阴沟肠杆菌、产气肠杆菌和科氏柠檬酸杆菌。详细的聚合酶链反应分析揭示了HPI的遗传组织存在差异；然而，在交叉喂养试验中，我们证明了耶尔森菌素的产生且该岛具有功能。
