Eyster L S, Van Camp L M
School of Biological Sciences, Flinders University, Adelaide, South Australia 5001, Australia.
Biol Bull. 2003 Aug;205(1):47-53. doi: 10.2307/1543444.
Cephalopod metabolism typically involves carbohydrates and proteins, so that the lipid content of the mantle and all internal organs except the digestive gland is very low. Despite clear evidence of nonlipoid metabolic trends in cephalopods, we observed extracellular spheres, or droplets, in the cecum and digestive gland of newly collected juvenile, male, and female individuals of Idiosepius notoides, the southern pygmy squid. Prior to staining, the droplets were various shades of yellow and were often large enough to detect at 7 x magnification. The droplets were less dense than water, hydrophobic, and sudanophilic, staining positively with Sudan III, Sudan IV, and Sudan Black B. We conclude that these spheres are lipid and that they derive from the squid's normal field diet. When newly collected squid were starved in the laboratory, the droplets disappeared in 7-8 d and then reappeared in the cecum about 3 h after feeding.
头足类动物的新陈代谢通常涉及碳水化合物和蛋白质，因此，除消化腺外，外套膜和所有内脏器官的脂质含量都非常低。尽管有明确证据表明头足类动物存在非脂质代谢趋势，但我们在新采集的南方侏儒乌贼（Idiosepius notoides）的幼体、雄性和雌性个体的盲肠和消化腺中观察到了细胞外球体或液滴。在染色之前，这些液滴呈现出各种深浅不一的黄色，并且通常大到在7倍放大倍数下就能检测到。这些液滴的密度比水小，具有疏水性且嗜苏丹染料，用苏丹III、苏丹IV和苏丹黑B染色呈阳性。我们得出结论，这些球体是脂质，并且它们来自鱿鱼的正常野外食物。当新采集的鱿鱼在实验室中饥饿时，这些液滴在7至8天内消失，然后在喂食后约3小时在盲肠中重新出现。
