Randall L P, Ridley A M, Cooles S W, Sharma M, Sayers A R, Pumbwe L, Newell D G, Piddock L J V, Woodward M J
Veterinary Laboratories Agency (Weybridge), New Haw, Addlestone, Surrey KT15 3NB, UK.
J Antimicrob Chemother. 2003 Sep;52(3):507-10. doi: 10.1093/jac/dkg379. Epub 2003 Aug 13.
In view of recent findings that a multidrug efflux pump CmeABC exists in Campylobacter jejuni, 391 C. jejuni and 52 Campylobacter coli of human and animal origin were examined for a multidrug resistance phenotype.
The MICs of ampicillin, chloramphenicol, ciprofloxacin, erythromycin, kanamycin, tetracycline, cetrimide, triclosan, acridine orange, paraquat and ethidium bromide were determined. Resistance to organic solvents and the effect of salicylate (known inducer of the marRAB operon in Escherichia coli and Salmonella) were also examined.
Two C. coli and 13 C. jejuni isolates, mainly from pigs or poultry, were resistant to three or more antibiotics and 12 of these strains had reduced susceptibility to acridine orange and/or ethidium bromide. Strains (n = 20) that were less susceptible to acridine orange, ethidium bromide and triclosan were significantly more resistant (P < 0.05) to ampicillin, chloramphenicol, ciprofloxacin, erythromycin, nalidixic acid and tetracycline, with two- to four-fold increases in MIC values compared with strains (n = 20) most susceptible to acridine orange, ethidium bromide and triclosan. Growth of strains with 1 mM salicylate caused a small (up to two-fold) but statistically significant (P < or = 0.005) increase in the MICs of chloramphenicol, ciprofloxacin, erythromycin and tetracycline.
These data indicate that multiple antibiotic resistant (MAR)-like Campylobacter strains occur and it may be postulated that these may overexpress cmeABC or another efflux system.
鉴于最近发现空肠弯曲菌中存在多药外排泵CmeABC，对391株来源于人和动物的空肠弯曲菌以及52株结肠弯曲菌进行了多药耐药表型检测。
测定了氨苄西林、氯霉素、环丙沙星、红霉素、卡那霉素、四环素、十六烷基三甲基溴化铵、三氯生、吖啶橙、百草枯和溴化乙锭的最低抑菌浓度（MIC）。还检测了对有机溶剂的抗性以及水杨酸盐（已知可诱导大肠杆菌和沙门氏菌中marRAB操纵子）的影响。
主要来自猪或家禽的2株结肠弯曲菌和13株空肠弯曲菌分离株对三种或更多种抗生素耐药，其中12株菌株对吖啶橙和/或溴化乙锭的敏感性降低。对吖啶橙、溴化乙锭和三氯生敏感性较低的菌株（n = 20）对氨苄西林、氯霉素、环丙沙星、红霉素、萘啶酸和四环素的耐药性显著更高（P < 0.05），与对吖啶橙、溴化乙锭和三氯生最敏感的菌株（n = 20）相比，MIC值增加了2至4倍。1 mM水杨酸盐处理的菌株生长导致氯霉素、环丙沙星、红霉素和四环素的MIC值有小幅（高达两倍）但具有统计学意义（P ≤ 0.005）的增加。
这些数据表明存在类似多重抗生素耐药（MAR）的弯曲菌菌株，可以推测这些菌株可能过度表达cmeABC或其他外排系统。
