Kousar Shaheena, Rehman Nikhar, Javed Asghar, Hussain Aamir, Naeem Muhammad, Masood Samra, Ali Hafiz Arslan, Manzoor Amna, Khan Aleem Ahmed, Akrem Ahmed, Iqbal Fatima, Zulfiqar Aqsa, Jamshaid Muhammad Bilal, Waqas Muhammad, Waseem Asma, Saeed Muhammad Qamar
Dr. Ghulam Nabi Chaudhry Laboratory of Microbial Technologies, Department of Microbiology and Molecular Genetics, Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, Pakistan.
Pathology Department, Nishtar Medical University, Multan, Pakistan.
Infect Drug Resist. 2021 Oct 29;14:4511-4516. doi: 10.2147/IDR.S324055. eCollection 2021.
The drastic increase in use of antibiotics as a mandatory part of production in poultry and livestock has led to the development of bacterial resistance against antibiotics. The spread of resistant bacteria from poultry to humans increases the risk of treatment failure by antibiotics because of resistance genes transfer.
The objective of the study was to estimate and compare the resistance profile collected from areas around the poultry farm premises and areas at least 500 meters away from the nearest poultry farm. We studied the effect of antibiotic usage in farms on the bacterial profile present in the upper layer of soil.
A total of 1,200 moist soil samples were collected from areas within a 25 meters range of poultry farms and areas that had no poultry farms in its 500 meters vicinity. was cultured and isolated. The antibiotic susceptibility profile was carried out by Kirby-Bauer disc diffusion method and results were analyzed according to CLSI guidelines. Statistical analysis was carried out to check the significance of results.
A total of 300 isolates were isolated, among which 140 isolates were isolated from areas around the poultry farm premises and had higher prevalence of antibiotic resistance. A total of 160 isolates were isolated from areas outside the poultry farm range. Resistance was not as high as in the isolates from around the farm. The ESBL production was higher in the isolates that were in close contact with the poultry farm as compared to the isolates away from the farm.
Use of antibiotics in the poultry farm for production significantly increases the resistance in bacterial strains present in the upper layer of soil around the poultry farm within at least a 25 meter range.
在家禽和家畜生产中强制使用抗生素的情况急剧增加，导致了细菌对抗生素产生耐药性。耐药细菌从家禽传播到人类，由于耐药基因的转移，增加了抗生素治疗失败的风险。
本研究的目的是估计和比较从家禽养殖场周边地区以及距离最近家禽养殖场至少500米的地区收集的耐药情况。我们研究了养殖场抗生素使用对土壤上层存在的细菌谱的影响。
总共从家禽养殖场25米范围内的区域以及500米范围内没有家禽养殖场的区域收集了1200份潮湿土壤样本。进行培养和分离。通过 Kirby-Bauer 纸片扩散法进行抗生素敏感性分析，并根据 CLSI 指南分析结果。进行统计分析以检验结果的显著性。
总共分离出300株菌株，其中140株从家禽养殖场周边地区分离得到，抗生素耐药性患病率较高。总共160株菌株从养殖场范围外的区域分离得到。耐药性不如养殖场周边分离株高。与远离养殖场的分离株相比，与家禽养殖场密切接触的分离株中ESBL的产生更高。
家禽养殖场用于生产的抗生素使用显著增加了至少25米范围内家禽养殖场周边土壤上层存在的细菌菌株的耐药性。
