Xu Hui, Sharma Ajay, Okabe Jeannine, Cui Cunqi, Huang Liping, Wei Yuan Yuan, Wan Hua, Lei Ying, Logan John S, Levy Marlon F, Byrne Guerard W
Nextran Inc., Princeton, NJ 08540, USA.
ASAIO J. 2003 Jul-Aug;49(4):407-16.
Improvements in xenotransplantation may significantly increase the availability of organs for human transplantation. The use of porcine organs, however, has raised concern about possible transmission of porcine endogenous retroviruses (PERV) to the recipients. The authors developed monoclonal antibodies specific to the PERV Gag viral product and show that these antibodies can detect PERV antigen under a variety of assay conditions, including enzyme linked immunosorbent assay (ELISA), Western blot, and immunofluorescence staining methods. Two patients in fulminant hepatic failure were treated by extracorporeal perfusion using transgenic porcine livers before receiving orthotopic liver transplants. Despite the use of immune suppression that allowed survival of the allograft, these patients both showed a strong immune response to the xenograft suggesting a largely intact capability to mount a humoral immune response. However, analysis of patient serum samples over a 3 to 4 year period has showed no evidence of an immune response to PERV antigens, suggesting a lack of PERV infection.
异种移植技术的改进可能会显著增加可用于人类移植的器官数量。然而，使用猪器官引发了人们对猪内源性逆转录病毒（PERV）可能传播给接受者的担忧。作者开发了针对PERV Gag病毒产物的单克隆抗体，并表明这些抗体可以在多种检测条件下检测到PERV抗原，包括酶联免疫吸附测定（ELISA）、蛋白质印迹法和免疫荧光染色法。两名暴发性肝衰竭患者在接受原位肝移植前，先用转基因猪肝脏进行体外灌注治疗。尽管使用了免疫抑制措施以使同种异体移植物存活，但这两名患者对异种移植物均表现出强烈的免疫反应，表明其产生体液免疫反应的能力基本完好。然而，对患者血清样本进行的3至4年分析显示，没有证据表明对PERV抗原存在免疫反应，这表明没有PERV感染。
