Alexeev Vladimir L, Sharma Anjal C, Goponenko Alexander V, Das Sasmita, Lednev Igor K, Wilcox Craig S, Finegold David N, Asher Sanford A
Department of Chemistry, Chevron Science Center, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA.
Anal Chem. 2003 May 15;75(10):2316-23. doi: 10.1021/ac030021m.
We demonstrate a colorimetric glucose recognition material consisting of a crystalline colloidal array embedded within a polyacrylamide-poly(ethylene glycol) (PEG) hydrogel, or a polyacrylamide-15-crown-5 hydrogel, with pendent phenylboronic acid groups. We utilize a new molecular recognition motif, in which boronic acid and PEG (or crown ether) functional groups are prepositioned in a photonic crystal hydrogel, such that glucose self-assembles these functional groups into a supramolecular complex. The formation of the complex results in an increase in the hydrogel cross-linking, which for physiologically relevant glucose concentration blue shifts the photonic crystal diffraction. The visually evident diffraction color shifts across the visible spectral region over physiologically important glucose concentration ranges. These materials respond to glucose at physiological ionic strengths and pH values and are selective in their mode of response for glucose over galactose, mannose, and fructose. Thus, we have developed a new recognition motif for glucose that shows promise for the fabrication of noninvasive or minimally invasive in vivo glucose sensing for patients with diabetes mellitus.
我们展示了一种比色葡萄糖识别材料，它由嵌入聚丙烯酰胺 - 聚乙二醇（PEG）水凝胶或聚丙烯酰胺 - 15 - 冠 - 5水凝胶中的结晶胶体阵列组成，这些水凝胶带有悬垂的苯基硼酸基团。我们利用了一种新的分子识别基序，其中硼酸和PEG（或冠醚）官能团预先定位在光子晶体水凝胶中，使得葡萄糖将这些官能团自组装成超分子复合物。复合物的形成导致水凝胶交联增加，对于生理相关的葡萄糖浓度，这会使光子晶体衍射发生蓝移。在生理上重要的葡萄糖浓度范围内，视觉上明显的衍射颜色在可见光谱区域发生变化。这些材料在生理离子强度和pH值下对葡萄糖有响应，并且在对葡萄糖的响应模式上对半乳糖、甘露糖和果糖具有选择性。因此，我们开发了一种新的葡萄糖识别基序，有望用于为糖尿病患者制造非侵入性或微创体内葡萄糖传感器。
