Hirsch L R, Jackson J B, Lee A, Halas N J, West J L
Department of Bioengineering, Rice University, P.O. Box 1892, Houston, Texas 77251-1892, USA.
Anal Chem. 2003 May 15;75(10):2377-81. doi: 10.1021/ac0262210.
A rapid immunoassay capable of detecting analyte within complex biological media without any sample preparation is described. This was accomplished using gold nanoshells, layered dielectric-metal nanoparticles whose optical resonance is a function of the relative size of its constituent layers. Aggregation of antibody/nanoshell conjugates with extinction spectra in the near-infrared was monitored spectroscopically in the presence of analyte. Successful detection of immunoglobulins was achieved in saline, serum, and whole blood. This system constitutes a simple immunoassay capable of detecting sub-nanogram-per-milliliter quantities of various analytes in different media within 10-30 min.
本文描述了一种无需任何样品制备即可在复杂生物介质中检测分析物的快速免疫测定方法。这是通过金纳米壳实现的，金纳米壳是一种层状介电-金属纳米颗粒，其光学共振是其组成层相对尺寸的函数。在存在分析物的情况下，通过光谱监测抗体/纳米壳缀合物在近红外区域的消光光谱的聚集情况。在盐水、血清和全血中成功检测到了免疫球蛋白。该系统构成了一种简单的免疫测定方法，能够在10 - 30分钟内检测出不同介质中每毫升亚纳克量的各种分析物。
