Yim Joung Han, Kim Sung Jin, Ahn Se Hoon, Lee Hong Kum
Microbiology Laboratory, Korea Ocean Research & Development Institute, Ansan P.O. Box 29, Seoul 425-600, Republic of Korea.
Biomol Eng. 2003 Jul;20(4-6):273-80. doi: 10.1016/s1389-0344(03)00070-4.
A marine microalga Gyrodinium impudicum strain KG03 produced sulfated exopolysaccharide designated as p-KG03, which showed a strong antiviral activity against encephalomyocarditis virus (EMCV). To optimize culture conditions for the production of p-KG03, mineral salts, vitamins, plant growth hormones, temperature, pH and light conditions were examined. From this study, M-KG03 medium for the maximum production of p-KG03 was suggested as follows; NH(4)Cl 75 microM, NaH(3)PO(4) 200 microM, NaHCO(3) 50 microM, Na(2)SO(4) 10 microM, FeCl(2) x 6H(2)O 10 microM, MnCl(2) x 4H(2)O 0.1 microM, vitamin B(12) 0.75 microg, naphthalene acetic acid (NAA) 7.5 microg and myo-inositol 200 mg per liter of aged sea water. The optimal temperature and pH were 22.5 degrees C and 8.0, respectively. The optimal light conditions of intensity and period were 150 microE m(-2) s(-1) and 16:8 h light:dark cycle. Finally, the cell growth and p-KG03 production were measured in one liter of M-KG03 medium with 1% CO(2) and 50 ml min(-1) of airflow using two liters airlift balloon type photobioreactor (ABTPR). At these optimal conditions, p-KG03 production and cell growth were 134.6+/-5.9 mg l(-1) and 123,076+/-1,597 cells ml(-1), respectively, representing a 7.7 and 5.1 times compared with f/2 medium with Erlenmeyer flask culture (p-KG03 production 17.5+/-1.3 mg l(-1) and cell growth 24,311+/-1,291 cells ml(-1)).
一种海洋微藻——裸甲藻属KG03菌株产生了一种名为p - KG03的硫酸化胞外多糖，该多糖对脑心肌炎病毒（EMCV）表现出强大的抗病毒活性。为了优化p - KG03的生产培养条件，对矿质盐、维生素、植物生长激素、温度、pH值和光照条件进行了研究。通过这项研究，建议用于最大量生产p - KG03的M - KG03培养基配方如下：每升老化海水中含有75微摩尔氯化铵、200微摩尔磷酸二氢钠、50微摩尔碳酸氢钠、10微摩尔硫酸钠、10微摩尔六水合氯化亚铁、0.1微摩尔四水合氯化锰、0.75微克维生素B12、7.5微克萘乙酸（NAA）和200毫克肌醇。最佳温度和pH值分别为22.5摄氏度和8.0。强度和周期的最佳光照条件分别为150微爱因斯坦·米⁻²·秒⁻¹和16:8小时的光暗循环。最后，使用两升气升式气球型光生物反应器（ABTPR），在含有1%二氧化碳和50毫升·分钟⁻¹气流的一升M - KG03培养基中测量细胞生长和p - KG03的产量。在这些最佳条件下，p - KG03的产量和细胞生长量分别为134.6±5.9毫克·升⁻¹和123,076±1,597个细胞·毫升⁻¹），与在锥形瓶中用f/2培养基培养相比，分别提高了7.7倍和5.1倍（p - KG03产量为17.5±1.3毫克·升⁻¹，细胞生长量为24,311±1,291个细胞·毫升⁻¹）。
