Yim Joung Han, Kim Sung Jin, Ahn Se Hun, Lee Chong Kyo, Rhie Ki Tae, Lee Hong Kum
Microbiology Laboratory, Korea Ocean Research & Development Institute, Ansan P.O. Box 29, Seoul 425-600, Republic of Korea.
Mar Biotechnol (NY). 2004 Jan-Feb;6(1):17-25. doi: 10.1007/s10126-003-0002-z. Epub 2003 Sep 29.
The sulfated exopolysaccharide p-KG03, which is produced by the marine microalga Gyrodinium impudicum strain KG03, exhibited impressive antiviral activity in vitro (EC50 = 26.9 micro g/ml) against the encephalomyocarditis virus (EMCV). Depending on the p-KG03 concentration, the development of cytopathic effects in EMCV-infected HeLa cells was either inhibited completely or slowed. Moreover, p-KG03 did not show any cytotoxic effects on HeLa cells, even at concentrations up to 1000 microg/ml. The polysaccharide was purified by repeated precipitation in ethanol, followed by gel filtration. The p-KG03 polysaccharide had a molecular weight of 1.87 x 10(7), and was characterized as a homopolysaccharide of galactose with uronic acid (2.96% wt/wt) and sulfate groups (10.32% wt/wt). The biological activities of p-KG03 suggest that sulfated metabolites from marine organisms are a rich source of antiviral agents. This is the first reported marine source of antiviral sulfated polysaccharides against EMCV. The p-KG03 polysaccharide may be useful in the development of marine bioactive exopolysaccharide for biotechnological and pharmaceutical products.
由海洋微藻裸甲藻菌株KG03产生的硫酸化胞外多糖p-KG03，在体外对脑心肌炎病毒（EMCV）表现出显著的抗病毒活性（EC50 = 26.9微克/毫升）。根据p-KG03的浓度，EMCV感染的HeLa细胞中细胞病变效应的发展要么被完全抑制，要么减缓。此外，即使在浓度高达1000微克/毫升时，p-KG03对HeLa细胞也未显示出任何细胞毒性作用。该多糖通过在乙醇中反复沉淀，然后进行凝胶过滤进行纯化。p-KG03多糖的分子量为1.87×10⁷，其特征为含有糖醛酸（2.96%重量/重量）和硫酸基团（10.32%重量/重量）的半乳糖同多糖。p-KG03的生物活性表明，海洋生物的硫酸化代谢产物是抗病毒剂的丰富来源。这是首次报道的针对EMCV的海洋来源抗病毒硫酸化多糖。p-KG03多糖可能有助于开发用于生物技术和医药产品的海洋生物活性胞外多糖。
