Chen Lu, El-Husseini Alaa, Tomita Susumu, Bredt David S, Nicoll Roger A
Department of Cellular and Molecular Pharmacology, University of California at San Francisco, San Francisco, CA 94143, USA.
Mol Pharmacol. 2003 Sep;64(3):703-6. doi: 10.1124/mol.64.3.703.
Synaptic plasticity at excitatory synapses in the brain is largely achieved by rapid changes in the number of synaptic alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate (AMPA) receptors. Stargazin, a membrane protein that interacts with AMPA receptors, is believed to play a pivotal role in trafficking AMPA receptors to the plasma membrane and targeting them to the synapse. However, it is unclear whether the trafficking of kainate receptors, which are structurally very similar to AMPA receptors, is also dependent on stargazin. Here we show that in both cerebellar granule cells and in Xenopus laevis oocytes expression system, surface delivery of kainate receptor is independent of stargazin. These results suggest that stargazin action is highly selective for AMPA receptors.
大脑中兴奋性突触的突触可塑性很大程度上是通过突触α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体数量的快速变化来实现的。Stargazin是一种与AMPA受体相互作用的膜蛋白，被认为在将AMPA受体转运到质膜并将其靶向突触方面发挥关键作用。然而，尚不清楚结构上与AMPA受体非常相似的海人藻酸受体的转运是否也依赖于Stargazin。在这里，我们表明，在小脑颗粒细胞和非洲爪蟾卵母细胞表达系统中，海人藻酸受体的表面递送均不依赖于Stargazin。这些结果表明，Stargazin的作用对AMPA受体具有高度选择性。
