Zhang Bingyong, Lu Yumin, Hong Tianpei, Pei Fei, Li Fuying, Wang Xingjuan
Department of Gastroenterology, Peking University Third Hospital, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2003 Apr 18;35(2):150-3.
To investigate the expression of interleukin 18 (IL-18) in intestinal mucosa of patients with inflammatory bowel disease (IBD) and its relation with disease active state.
IL-18mRNA transcripts were evaluated by using a semiquantitative RT-PCR protocol. Specimens of 23 Crohn's disease (CD) and 20 ulcerative colitis (UC) patients and 20 controls were studied by immunohistochemical staining.
Transcripts of IL-18 were found to be increased significantly in intestinal mucosa from active CD compared with UC and control. Immunohistochemical staining analysis localized the expression of IL-18 to intestinal epithelial cells and lamina propria (macrophages and dendritic cells, mainly). Staining was more intense in active CD compared with UC and control. Both transcriptions and staining of IL-18 in active UC were elevated, but there was no significant difference compared with control. Expression of IL-18 decreased in inactive CD (n = 9) or UC (n = 11) compared with active state of the same patients, and the difference in CD had significance.
IL-18 may be involved in the pathogenesis of IBD and related with CD activity.
探讨白细胞介素18（IL-18）在炎症性肠病（IBD）患者肠黏膜中的表达及其与疾病活动状态的关系。
采用半定量逆转录聚合酶链反应（RT-PCR）方法评估IL-18mRNA转录本。通过免疫组织化学染色研究23例克罗恩病（CD）患者、20例溃疡性结肠炎（UC）患者以及20例对照者的标本。
发现与UC和对照相比，活动期CD患者肠黏膜中IL-18转录本显著增加。免疫组织化学染色分析显示IL-18表达定位于肠上皮细胞和固有层（主要是巨噬细胞和树突状细胞）。与UC和对照相比，活动期CD的染色更强。活动期UC中IL-18的转录和染色均升高，但与对照相比无显著差异。与同一患者的活动期相比，非活动期CD（n = 9）或UC（n = 11）中IL-18的表达降低，且CD中的差异具有统计学意义。
IL-18可能参与IBD的发病机制，并与CD活动相关。
