Abba M C, Gomez M A, Golijow C D
Centro de Investigaciones en Genética Básica y Aplicada (CIGEBA), Facultad de Ciencias Veterinarias, Universidad Nacional de La Plata, calle 60 y 118 s/n, CC 296, B1900AVW La Plata, Buenos Aires, Argentina.
Rev Argent Microbiol. 2003 Apr-Jun;35(2):74-9.
Genital infection with human papillomavirus (HPV) is one of the most common sexually transmitted viral diseases. High risk HPV are now considered the main etiologic agent of cancer of the uterine cervix and their high-grade precursor lesions. The aim of the present study was to investigate the endemic HPV-genotype spectrum in a population of women from the city of La Plata, Argentina. With this purpose, 718 cervical scrapes or biopsies corresponding to 152 normal samples (Pap I/II), 84 samples classified as atypical squamous cells of undetermined significance (ASCUS), 100 condyloma, 279 low-grade squamous intraepithelial lesions (LGSIL), 82 high-grade squamous intraepithelial lesions (HGSIL), and 21 squamous cell carcinomas (SCC) were studied. The detection of HPV-DNA was performed by nested polymerase chain reaction, using My 09/11 and Gp 05/06. The viral genotypes were analyzed by single-stranded conformation polymorphisms, employing low ionic strength solution (LIS-SSCP). The overall prevalence of HPV infection was 75% in the analyzed population, with a frequency of 46% for normal cervix, 69% for ASCUS, 86% for condyloma, 80% for LGSIL, 98% for HGSIL and 100% for SCC. The most prevalent viral types were HPV 16 (35%), followed by HPV 6/11 (27% each one), HPV 33 (6%) and HPV 18 (5%). HPV 16 was the most prevalent viral type among women with LGSIL, HGSIL and SCC, representing 33%, 50% and 67% of the genital infections, respectively. HPV 6 and 11 were the most frequent viral types among samples classified as Pap I/II, ASCUS and condyloma. Women between 21 and 30 year old showed the highest prevalence of HPV positivity, compraising the 32.2% of total infections.
人乳头瘤病毒（HPV）引起的生殖器感染是最常见的性传播病毒性疾病之一。高危型HPV目前被认为是子宫颈癌及其高级别前驱病变的主要病因。本研究的目的是调查阿根廷拉普拉塔市女性人群中HPV地方流行基因型谱。为此，研究了718份宫颈刮片或活检样本，分别对应152份正常样本（巴氏I/II级）、84份意义不明确的非典型鳞状细胞样本（ASCUS）、100份尖锐湿疣样本、279份低级别鳞状上皮内病变（LGSIL）样本、82份高级别鳞状上皮内病变（HGSIL）样本以及21份鳞状细胞癌（SCC）样本。采用My 09/11和Gp 05/06引物，通过巢式聚合酶链反应检测HPV-DNA。采用低离子强度溶液（LIS-SSCP）通过单链构象多态性分析病毒基因型。在所分析人群中，HPV感染的总体患病率为75%，正常宫颈的感染率为46%，ASCUS为69%，尖锐湿疣为86%，LGSIL为80%，HGSIL为98%，SCC为100%。最常见的病毒类型为HPV 16（35%），其次是HPV 6/11（各占27%）、HPV 33（6%）和HPV 18（5%）。HPV 16是LGSIL、HGSIL和SCC女性中最常见的病毒类型，分别占生殖器感染的33%、50%和67%。HPV 6和11是巴氏I/II级、ASCUS和尖锐湿疣样本中最常见的病毒类型。21至30岁的女性HPV阳性患病率最高，占总感染数的32.2%。
