Grce M, Husnjak K, Bozikov J, Magdić L, Zlacki M, Lukac J, Fistonić I, Sikanić-Dugic N, Pavelić K
Rudjer Bosković Institute, Division of Molecular Medicine, Bijenicka 54, 10000 Zagreb, Croatia.
Anticancer Res. 2001 Jan-Feb;21(1B):579-84.
Infection with specific human papillomavirus (HPV) types is the strongest risk factor in cervical carcinogenesis. In this study we analysed, by means of polymerase chain reaction (PCR), cervical specimens obtained from consenting women with abnormal Pap smears collected from 1996 to 1998. Consensus- and type-specific-primers directed PCR were used in order to detect the presence and to determine the most common HPV types: 6, 11, 16, 18, 31 and 33. Out of 1874 specimens, 1207 (64%) contained one or more HPV types. Approximately half HPVs were typed (621 out of 1207) and the others remained untyped (586 out of 1207), 51% and 49%, respectively. Beside low-risk HPV 6/11 (5%), the most frequently observed HPVs were high-risk HPV types, especially type 16 (12%), while HPV types 18 (2%), 31 (5%) and 33 (3%) were less frequent. The HPV positivity rate declined with age, although all HPV types were equally distributed in different age groups. The presence of HPV DNA significantly increased from 55% to 78% along with the severity of the cervical lesions, i.e. low- and high-grade squamous intraepithelial lesions (LSIL, HSIL). Undetermined HPV types, other than 6/11, 16, 18, 31 and 33 were equally distributed in LSIL and HSIL which indicates that they represent low- as well as high-risk HPV types. Our results indicated that HPV infections, especially those with HPV 16, represent a significant public health concern in Croatia.
感染特定类型的人乳头瘤病毒（HPV）是宫颈癌发生的最强风险因素。在本研究中，我们通过聚合酶链反应（PCR）分析了1996年至1998年期间从同意参与研究的巴氏涂片异常的女性中获取的宫颈标本。使用共识引物和型特异性引物进行PCR，以检测HPV的存在并确定最常见的HPV类型：6、11、16、18、31和33型。在1874份标本中，1207份（64%）含有一种或多种HPV类型。大约一半的HPV被分型（1207份中的621份），其余的未分型（1207份中的586份），分别占51%和49%。除了低风险的HPV 6/11型（5%）外，最常检测到的HPV是高风险类型，尤其是16型（12%），而18型（2%）、31型（5%）和33型（3%）则较少见。HPV阳性率随年龄下降，尽管所有HPV类型在不同年龄组中的分布是均匀的。随着宫颈病变的严重程度增加，即低级别和高级别鳞状上皮内病变（LSIL、HSIL），HPV DNA的检出率从55%显著增加到78%。除6/11、16、18、31和33型外，未确定类型的HPV在LSIL和HSIL中分布均匀，这表明它们既代表低风险也代表高风险HPV类型。我们的结果表明，HPV感染，尤其是HPV 16感染，在克罗地亚是一个重大的公共卫生问题。
