枯草芽孢杆菌中ClpCP的独特降解信号。
Unique degradation signal for ClpCP in Bacillus subtilis.
作者信息
Pan Qi, Losick Richard
机构信息
Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA.
出版信息
J Bacteriol. 2003 Sep;185(17):5275-8. doi: 10.1128/JB.185.17.5275-5278.2003.
Abstract
Regulation of the cell-specific transcription factor sigma(F) in the spore-forming bacterium Bacillus subtilis involves the antisigma factor SpoIIAB. Contributing to the activation of sigma(F) is the degradation of SpoIIAB in a manner that depends on the protease ClpCP. Here we show that the three residues (LCN) located at the extreme C terminus of SpoIIAB are both necessary and sufficient for this degradation. We also report that the use of the LCN extension as a degradation signal for ClpCP is unique to SpoIIAB.
摘要
在形成芽孢的细菌枯草芽孢杆菌中，细胞特异性转录因子σ(F)的调控涉及抗σ因子SpoIIAB。SpoIIAB以依赖蛋白酶ClpCP的方式降解，这有助于σ(F)的激活。我们在此表明，位于SpoIIAB极端C末端的三个残基（LCN）对于这种降解既是必需的也是充分的。我们还报告说，将LCN延伸用作ClpCP的降解信号是SpoIIAB所特有的。
相似文献
1
Unique degradation signal for ClpCP in Bacillus subtilis.枯草芽孢杆菌中ClpCP的独特降解信号。
J Bacteriol. 2003 Sep;185(17):5275-8. doi: 10.1128/JB.185.17.5275-5278.2003.
2
Self-reinforcing activation of a cell-specific transcription factor by proteolysis of an anti-sigma factor in B. subtilis.枯草芽孢杆菌中通过抗σ因子的蛋白水解作用实现细胞特异性转录因子的自我强化激活。
Mol Cell. 2001 Oct;8(4):873-83. doi: 10.1016/s1097-2765(01)00362-8.
3
SpoIIAB is an anti-sigma factor that binds to and inhibits transcription by regulatory protein sigma F from Bacillus subtilis.SpoIIAB是一种抗σ因子，它能结合并抑制来自枯草芽孢杆菌的调控蛋白σF的转录作用。
Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2325-9. doi: 10.1073/pnas.90.6.2325.
4
Polar localization and compartmentalization of ClpP proteases during growth and sporulation in Bacillus subtilis.枯草芽孢杆菌生长和芽孢形成过程中ClpP蛋白酶的极性定位和区室化
J Bacteriol. 2008 Oct;190(20):6749-57. doi: 10.1128/JB.00589-08. Epub 2008 Aug 8.
5
Chaperone-protease systems in regulation and protein quality control in Bacillus subtilis.枯草芽孢杆菌中伴侣蛋白酶系统在调控和蛋白质质量控制中的作用。
Res Microbiol. 2009 Nov;160(9):637-44. doi: 10.1016/j.resmic.2009.08.020. Epub 2009 Sep 23.
6
MurAA, catalysing the first committed step in peptidoglycan biosynthesis, is a target of Clp-dependent proteolysis in Bacillus subtilis.MurAA催化肽聚糖生物合成中的第一步关键反应，是枯草芽孢杆菌中Clp依赖性蛋白酶解作用的一个靶点。
Mol Microbiol. 2004 Feb;51(4):1087-102. doi: 10.1046/j.1365-2958.2003.03875.x.
7
Molecular determinants of MecA as a degradation tag for the ClpCP protease.作为ClpCP蛋白酶降解标签的MecA的分子决定因素。
J Biol Chem. 2009 Dec 4;284(49):34366-75. doi: 10.1074/jbc.M109.053017. Epub 2009 Sep 18.
8
Regulation by proteolysis in bacterial cells.细菌细胞中通过蛋白水解作用进行的调控。
Curr Opin Microbiol. 2003 Apr;6(2):163-72. doi: 10.1016/s1369-5274(03)00029-8.
9
Forespore-specific transcription of the lonB gene during sporulation in Bacillus subtilis.枯草芽孢杆菌芽孢形成过程中lonB基因的前芽孢特异性转录。
J Bacteriol. 2001 May;183(10):2995-3003. doi: 10.1128/JB.183.10.2995-3003.2001.
10
Involvement of Bacillus subtilis ClpE in CtsR degradation and protein quality control.枯草芽孢杆菌ClpE参与CtsR降解及蛋白质质量控制。
J Bacteriol. 2006 Jul;188(13):4610-9. doi: 10.1128/JB.00287-06.
引用本文的文献
1
The final proteolytic step in transmembrane signaling of multiple RsgI anti-σ factors in Clostridium thermocellum.嗜热栖热放线菌中多种RsgI抗σ因子跨膜信号传导的最终蛋白水解步骤。
Biosci Rep. 2025 Apr 9;45(4):233-45. doi: 10.1042/BSR20253055.
2
ClpX/P-Dependent Degradation of Novel Substrates in Streptococcus mutans.变形链球菌 ClpX/P 依赖性新型底物的降解。
J Bacteriol. 2022 Apr 19;204(4):e0059421. doi: 10.1128/jb.00594-21. Epub 2022 Mar 28.
3
Protein degradation control and regulation of bacterial survival and pathogenicity: the role of protein degradation systems in bacteria.蛋白质降解对细菌存活和致病性的控制与调节：蛋白质降解系统在细菌中的作用
Mol Biol Rep. 2021 Nov;48(11):7575-7585. doi: 10.1007/s11033-021-06744-9. Epub 2021 Oct 15.
4
A nutrient-dependent division antagonist is regulated post-translationally by the Clp proteases in Bacillus subtilis.枯草芽孢杆菌中 Clp 蛋白酶通过翻译后调控营养依赖性分裂抑制剂。
BMC Microbiol. 2018 Apr 6;18(1):29. doi: 10.1186/s12866-018-1155-2.
5
The C-Terminal Region of Bacillus subtilis SwrA Is Required for Activity and Adaptor-Dependent LonA Proteolysis.枯草芽孢杆菌 SwrA 的 C 端区域对于活性和衔接蛋白依赖性 LonA 蛋白水解是必需的。
J Bacteriol. 2018 Feb 23;200(6). doi: 10.1128/JB.00659-17. Print 2018 Mar 15.
6
A leptospiral AAA+ chaperone-Ntn peptidase complex, HslUV, contributes to the intracellular survival of Leptospira interrogans in hosts and the transmission of leptospirosis.一个钩端螺旋体 AAA+ 伴侣-Ntn 肽酶复合物 HslUV，有助于钩端螺旋体在宿主细胞内的存活和钩端螺旋体病的传播。
Emerg Microbes Infect. 2017 Nov 29;6(11):e105. doi: 10.1038/emi.2017.93.
7
Functional Diversity of AAA+ Protease Complexes in .……中AAA+蛋白酶复合体的功能多样性
Front Mol Biosci. 2017 Jul 12;4:44. doi: 10.3389/fmolb.2017.00044. eCollection 2017.
8
Strain-Dependent Recognition of a Unique Degradation Motif by ClpXP in .ClpXP对独特降解基序的菌株依赖性识别 于……中
mSphere. 2016 Dec 7;1(6). doi: 10.1128/mSphere.00287-16. eCollection 2016 Nov-Dec.
9
YodL and YisK Possess Shape-Modifying Activities That Are Suppressed by Mutations in Bacillus subtilis mreB and mbl.YodL和YisK具有形状修饰活性，该活性在枯草芽孢杆菌mreB和mbl的突变中受到抑制。
J Bacteriol. 2016 Jul 13;198(15):2074-88. doi: 10.1128/JB.00183-16. Print 2016 Aug 1.
10
clpC operon regulates cell architecture and sporulation in Bacillus anthracis.clpC操纵子调控炭疽芽孢杆菌的细胞结构和孢子形成。
Environ Microbiol. 2015 Mar;17(3):855-65. doi: 10.1111/1462-2920.12548. Epub 2014 Jul 17.
本文引用的文献
1
Evidence in support of a docking model for the release of the transcription factor sigma F from the antisigma factor SpoIIAB in Bacillus subtilis.支持枯草芽孢杆菌中转录因子σF从抗σ因子SpoIIAB释放的对接模型的证据。
J Biol Chem. 2003 Jun 6;278(23):20898-905. doi: 10.1074/jbc.M302305200. Epub 2003 Apr 3.
2
The CtrA response regulator essential for Caulobacter crescentus cell-cycle progression requires a bipartite degradation signal for temporally controlled proteolysis.对于新月柄杆菌细胞周期进程至关重要的CtrA反应调节因子需要一个双组分降解信号来进行时间控制的蛋白水解。
J Mol Biol. 2002 Nov 29;324(3):443-55. doi: 10.1016/s0022-2836(02)01042-2.
3
AAA+ proteins and substrate recognition, it all depends on their partner in crime.AAA+蛋白与底物识别，这完全取决于它们的“犯罪同伙”。
FEBS Lett. 2002 Oct 2;529(1):6-10. doi: 10.1016/s0014-5793(02)03179-4.
4
Multiple pathways of Spx (YjbD) proteolysis in Bacillus subtilis.枯草芽孢杆菌中Spx（YjbD）蛋白水解的多种途径。
J Bacteriol. 2002 Jul;184(13):3664-70. doi: 10.1128/JB.184.13.3664-3670.2002.
5
Differential gene expression governed by chromosomal spatial asymmetry.由染色体空间不对称性所调控的差异基因表达。
Cell. 2001 Nov 2;107(3):339-46. doi: 10.1016/s0092-8674(01)00528-1.
6
Self-reinforcing activation of a cell-specific transcription factor by proteolysis of an anti-sigma factor in B. subtilis.枯草芽孢杆菌中通过抗σ因子的蛋白水解作用实现细胞特异性转录因子的自我强化激活。
Mol Cell. 2001 Oct;8(4):873-83. doi: 10.1016/s1097-2765(01)00362-8.
7
The ATP-dependent HslVU/ClpQY protease participates in turnover of cell division inhibitor SulA in Escherichia coli.ATP 依赖性 HslVU/ClpQY 蛋白酶参与大肠杆菌中细胞分裂抑制剂 SulA 的周转。
J Bacteriol. 1999 Jun;181(12):3674-80. doi: 10.1128/JB.181.12.3674-3680.1999.
8
New insights into the ATP-dependent Clp protease: Escherichia coli and beyond.对ATP依赖型Clp蛋白酶的新见解：从大肠杆菌到其他生物
Mol Microbiol. 1999 May;32(3):449-58. doi: 10.1046/j.1365-2958.1999.01357.x.
9
Transient gene asymmetry during sporulation and establishment of cell specificity in Bacillus subtilis.枯草芽孢杆菌孢子形成过程中的瞬时基因不对称性及细胞特异性的建立
Genes Dev. 1999 Feb 15;13(4):394-9. doi: 10.1101/gad.13.4.394.
10
CtsR, a novel regulator of stress and heat shock response, controls clp and molecular chaperone gene expression in gram-positive bacteria.CtsR是应激和热休克反应的一种新型调节因子，可控制革兰氏阳性菌中clp和分子伴侣基因的表达。
Mol Microbiol. 1999 Jan;31(1):117-31. doi: 10.1046/j.1365-2958.1999.01152.x.
Zotero 插件