Bae Jin-Wan, Takahashi Ichiro, Sasano Yasuyuki, Onodera Kazuyuki, Mitani Hidetoshi, Kagayama Manabu, Mitani Hideo
Division of Orthodontics and Dentofacial Orthopedics, Tohoku University Graduate School of Dentistry, 4-1, Seiryo-machi, Aoba-ku, Sendai, Japan, 980-8575.
J Anat. 2003 Aug;203(2):235-41. doi: 10.1046/j.1469-7580.2003.00196.x.
Matrix metalloproteinases (MMPs) have been implicated in physiological cartilage matrix remodelling as well as in pathological and invasive extracellular matrix remodelling of tissue. Age-related changes in the gene expression patterns of MMPs in mandibular condylar cartilages (MCCs) were analysed. We examined the gene expression patterns of Mmp-8 and -13 and their substrates, Col1a1, Col2a1 and Col10a1, in MCC of growing and ageing rats. Temporomandibular joints of male Wistar rats aged 4, 8, 16 and 32 weeks were subjected to in situ hybridization analysis. Histologically, MMCs showed characteristics of growth plate cartilage at ages 4 and 8 weeks, and more closely resembled articular cartilage thereafter. Mmp-8 was expressed in the cells in all cartilaginous cell layers at ages 4 and 8 weeks, and then was localized only in the mature cells at ages 16 and 32 weeks. Whereas Mmp-13 expression was limited to the lowermost hypertrophic chondrocytes in the growth stage, mature chondrocytes instead of hypertrophic chondrocytes expressed Mmp-13 in adult non-hypertrophic MCC. Because Mmp-8 and -13 expression overlapped with Col2a1 and Col10a1, chondrocytes could play a pivotal role in degradation as well as production of the cartilaginous matrix in MCC.
基质金属蛋白酶（MMPs）与生理性软骨基质重塑以及组织的病理性和侵袭性细胞外基质重塑有关。分析了下颌髁突软骨（MCCs）中MMPs基因表达模式的年龄相关变化。我们检测了生长和衰老大鼠MCC中Mmp - 8和 - 13及其底物Col1a1、Col2a1和Col10a1的基因表达模式。对4周、8周、16周和32周龄雄性Wistar大鼠的颞下颌关节进行原位杂交分析。组织学上，4周和8周龄时MCCs表现出生长板软骨的特征，此后更类似于关节软骨。4周和8周龄时，Mmp - 8在所有软骨细胞层的细胞中表达，然后在16周和32周龄时仅定位于成熟细胞。而Mmp - 13的表达在生长阶段局限于最底层的肥大软骨细胞，在成年非肥大MCC中，成熟软骨细胞而非肥大软骨细胞表达Mmp - 13。由于Mmp - 8和 - 13的表达与Col2a1和Col10a1重叠，软骨细胞在MCC软骨基质的降解和产生中可能起关键作用。
