Department of Morphology and Genetics, Universidade Federal de São Paulo, São Paulo, São Paulo, Brazil.
Department of Neurology and Neurosurgery, Universidade Federal de São Paulo, São Paulo, São Paulo, Brazil.
PLoS One. 2020 Mar 12;15(3):e0230228. doi: 10.1371/journal.pone.0230228. eCollection 2020.
This study was designed to evaluate the anti-inflammatory effects of a curcumin treatment on the knee of rats with induced osteoarthritis. Fifteen adult rats were used and divided in three groups: the osteoarthritis group (OAG), control group (CG-without induction of osteoarthritis), and curcumin-treated osteoarthritis group (COAG). Osteoarthritis was induced in the right knee of rats in the OAG and COAG by administering an intra-articular injection of 1 mg of zymosan. Fourteen days after induction, 50 mg/kg curcumin was administered by gavage daily for 60 days to the COAG. After the treatment period, rats from all groups were euthanized. Medial femoral condyles were collected for light microscopy and immunohistochemical staining. The expression of SOX-5, IHH, MMP-8, MMP-13, and collagen 2 (Col2) was analyzed. The COAG exhibited an increase in the number of chondrocytes in the surface and middle layers compared with that of the OAG and CG, respectively. The COAG also showed a decrease in the thicknesses of the middle and deep layers compared with those of the OAG, and an increase in Col2 expression was observed in all articular layers (surface, middle, and deep) in the COAG compared with that in the OAG. SOX-5 expression was increased in the surface and deep layers of the COAG compared with those in the OAG and CG. Based on the results of this study, the curcumin treatment appeared to exert a protective effect on cartilage, as it did not result in an increase in cartilage thickness or in MMP-8 and MMP-13 expression but led to increased IHH, Col2, and SOX-5 expression and the number of chondrocytes.
本研究旨在评估姜黄素治疗对诱导性骨关节炎大鼠膝关节的抗炎作用。使用了 15 只成年大鼠，并将其分为三组：骨关节炎组（OAG）、对照组（CG-未诱导骨关节炎）和姜黄素治疗性骨关节炎组（COAG）。在 OAG 和 COAG 大鼠的右膝关节中通过关节内注射 1mg 酵母聚糖诱导骨关节炎。诱导后 14 天，COAG 每天通过灌胃给予 50mg/kg 姜黄素，持续 60 天。治疗期结束后，所有组别的大鼠均被安乐死。收集内侧股骨髁进行光镜和免疫组织化学染色。分析 SOX-5、IHH、MMP-8、MMP-13 和胶原 2（Col2）的表达。与 OAG 和 CG 相比，COAG 的表面和中层的软骨细胞数量增加。COAG 的中层和深层厚度也比 OAG 减少，并且在 COAG 的所有关节层（表面、中层和深层）中观察到 Col2 表达增加，而在 OAG 中观察到 SOX-5 表达增加。与 OAG 和 CG 相比，COAG 的表面和深层的 SOX-5 表达增加。根据本研究的结果，姜黄素治疗似乎对软骨具有保护作用，因为它不会导致软骨厚度增加或 MMP-8 和 MMP-13 表达增加，而是导致 IHH、Col2 和 SOX-5 表达增加以及软骨细胞数量增加。
