抗氧化剂对大鼠肠黏膜缺血再灌注诱导的细胞凋亡的影响。
Effects of antioxidative agents on apoptosis induced by ischaemia-reperfusion in rat intestinal mucosa.
作者信息
Kojima M, Iwakiri R, Wu B, Fujise T, Watanabe K, Lin T, Amemori S, Sakata H, Shimoda R, Oguzu T, Ootani A, Tsunada S, Fujimoto K
机构信息
Department of Internal Medicine and Gastrointestinal Endoscopy, Saga Medical School, Saga, Japan.
出版信息
Aliment Pharmacol Ther. 2003 Jul;18 Suppl 1:139-45. doi: 10.1046/j.1365-2036.18.s1.16.x.
BACKGROUND AND AIM
We have previously demonstrated that ischaemia-reperfusion induces apoptosis in the intestinal mucosa. To evaluate that reactive oxygen species enhanced intestinal apoptosis after ischaemia-reperfusion, we examined whether antioxidants reduced apoptosis.
METHODS
Rats were infused through a duodenal tube with antioxidative agents, glutathione, rebamipide and dymethylsulfoxide during 2 h before an ischaemic insult. The superior mesenteric artery was occluded for 60 min, followed by 60 min reperfusion. Apoptosis was evaluated by percentage fragmented DNA (fragmented DNA/total DNA) and immunochemical staining.
RESULTS
Increase in apoptosis in the intestinal mucosa after ischaemia-reperfusion was attenuated by intraduodenal infusion of antioxidative agents, but was not completely abolished.
CONCLUSION
Scavenging effects of the antioxidative agents attenuated increases in intestinal apoptosis, indicating that oxidative stress after ischaemia-reperfusion plays an important role in induction of apoptosis in the intestinal mucosa.
背景与目的
我们之前已经证明，缺血再灌注可诱导肠黏膜细胞凋亡。为评估活性氧是否会增强缺血再灌注后的肠道细胞凋亡，我们检测了抗氧化剂是否能减少细胞凋亡。
方法
在缺血损伤前2小时，通过十二指肠管向大鼠输注抗氧化剂、谷胱甘肽、瑞巴派特和二甲亚砜。肠系膜上动脉闭塞60分钟，随后再灌注60分钟。通过DNA片段化百分比（片段化DNA/总DNA）和免疫化学染色评估细胞凋亡。
结果
十二指肠内输注抗氧化剂可减轻缺血再灌注后肠黏膜细胞凋亡的增加，但并未完全消除。
结论
抗氧化剂的清除作用减弱了肠道细胞凋亡的增加，表明缺血再灌注后的氧化应激在肠黏膜细胞凋亡的诱导中起重要作用。
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