在大鼠中，地氟烷比氟烷对局灶性脑缺血具有更强的保护作用。

Desflurane affords greater protection than halothane against focal cerebral ischaemia in the rat.

作者信息

Haelewyn B, Yvon A, Hanouz J L, MacKenzie E T, Ducouret P, Gérard J L, Roussel S

机构信息

Laboratory of Experimental Anaesthesiology and Cellular Physiology, University of Caen, UPRES EA 3212, Département d'Anesthésie Réanimation, Centre Hospitalier Universitaire (CHU), Côte de Nacre, Caen, France.

出版信息

Br J Anaesth. 2003 Sep;91(3):390-6. doi: 10.1093/bja/aeg186.

DOI:10.1093/bja/aeg186

PMID:12925480

Abstract

BACKGROUND

We studied the potential neuroprotective effects of halothane and desflurane, compared with the awake state, on infarct size following 2 h of intraluminal middle cerebral artery occlusion (MCAo) and 22 h of reperfusion.

METHODS

Male Sprague-Dawley rats were anaesthetized with desflurane or halothane, intubated, and mechanically ventilated. Mean arterial pressure (MAP), blood gases, and pH were controlled. Body temperature was maintained at 37.5-38 degrees C. Animals were assigned to one of four groups according to the anaesthetic type (halothane or desflurane) and the duration of anaesthesia: "short-duration", during the preparation only; "long-duration", during both preparation and ischaemia. Twenty-four hours after MCAo, infarcts were visualized by staining with 2,3,5-triphenyltetrazolium chloride. Two additional groups of rats were subjected to the same protocol as that of long-duration halothane and long-duration desflurane with additional pericranial temperature measurements made.

RESULTS

Physiological parameters were comparable between the groups but MAP was higher (P<0.0001) in the short-duration groups. In the short-duration groups, cerebral infarct volumes were not significantly different between anaesthetics (short-duration halothane: 288 (61) mm(3), mean (SD); short-duration desflurane: 269 (71) mm(3), P>0.56). Compared with the awake state (short-duration groups), halothane and desflurane significantly reduced infarct volumes (long-duration halothane: 199 (54) mm(3), P<0.0047 vs short-duration halothane; long-duration desflurane: 121 (55) mm(3), P<0.0001 vs short-duration desflurane). The mean infarct volume in the long-duration desflurane group was significantly lower than that in the long-duration halothane group (P<0.0053). Pericranial temperatures were similar in the desflurane and halothane long-duration groups (P>0.17).

CONCLUSIONS

In rats, desflurane-induced neuroprotection against focal cerebral ischaemia was greater than that conferred by halothane.

摘要

背景

我们研究了与清醒状态相比，在大脑中动脉腔内闭塞（MCAo）2小时及再灌注22小时后，氟烷和地氟烷的潜在神经保护作用对梗死体积的影响。

方法

雄性Sprague-Dawley大鼠用氟烷或地氟烷麻醉，插管并进行机械通气。控制平均动脉压（MAP）、血气和pH值。体温维持在37.5-38摄氏度。根据麻醉类型（氟烷或地氟烷）和麻醉持续时间，将动物分为四组之一：“短时间组”，仅在准备过程中；“长时间组”，在准备和缺血过程中。MCAo后24小时，用2,3,5-三苯基氯化四氮唑染色观察梗死灶。另外两组大鼠采用与长时间氟烷和长时间地氟烷相同的方案，并额外测量颅周温度。

结果

各组间生理参数具有可比性，但短时间组的MAP较高（P<0.0001）。在短时间组中，不同麻醉剂之间的脑梗死体积无显著差异（短时间氟烷组：288（61）mm³，均值（标准差）；短时间地氟烷组：269（71）mm³，P>0.56）。与清醒状态（短时间组）相比，氟烷和地氟烷显著降低了梗死体积（长时间氟烷组：199（54）mm³，与短时间氟烷组相比P<0.004；长时间地氟烷组：121（55）mm³，与短时间地氟烷组相比P<0.0001）。长时间地氟烷组的平均梗死体积显著低于长时间氟烷组（P<0.0053）。地氟烷和氟烷长时间组的颅周温度相似（P>0.17）。