通过剪接体介导的RNA反式剪接进行信使核糖核酸重编程
Messenger RNA reprogramming by spliceosome-mediated RNA trans-splicing.
作者信息
Garcia-Blanco Mariano A
机构信息
Department of Molecular Genetics, Duke University Medical Center, Durham, North Carolina 27710, USA.
出版信息
J Clin Invest. 2003 Aug;112(4):474-80. doi: 10.1172/JCI19462.
Abstract
In the human genome, the majority of protein-encoding genes are interrupted by introns, which are removed from primary transcripts by a macromolecular enzyme known as the spliceosome. Spliceosomes can constitutively remove all the introns in a primary transcript to yield a fully spliced mRNA or alternatively splice primary transcripts leading to the production of many different mRNAs from one gene. This review examines how spliceosomes can recombine two primary transcripts in trans to reprogram messenger RNAs.
摘要
在人类基因组中，大多数蛋白质编码基因被内含子打断，这些内含子通过一种称为剪接体的大分子酶从初级转录本中去除。剪接体可以组成性地去除初级转录本中的所有内含子，以产生完全剪接的mRNA，或者选择性地剪接初级转录本，从而从一个基因产生许多不同的mRNA。这篇综述探讨了剪接体如何在反式中重组两个初级转录本以重新编程信使RNA。
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本文引用的文献
1
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Nat Med. 2003 Aug;9(8):1015-9. doi: 10.1038/nm900. Epub 2003 Jul 6.
2
Promoter choice determines splice site selection in protocadherin alpha and gamma pre-mRNA splicing.启动子的选择决定了原钙黏蛋白α和γ前体mRNA剪接中的剪接位点选择。
Mol Cell. 2002 Jul;10(1):21-33. doi: 10.1016/s1097-2765(02)00578-6.
3
Emerging clinical applications of RNA.RNA的新兴临床应用。
Nature. 2002 Jul 11;418(6894):252-8. doi: 10.1038/418252a.
4
An extensive network of coupling among gene expression machines.基因表达机制之间广泛的耦合网络。
Nature. 2002 Apr 4;416(6880):499-506. doi: 10.1038/416499a.
5
Partial correction of endogenous DeltaF508 CFTR in human cystic fibrosis airway epithelia by spliceosome-mediated RNA trans-splicing.通过剪接体介导的RNA反式剪接对人类囊性纤维化气道上皮细胞中的内源性DeltaF508囊性纤维化跨膜传导调节因子进行部分校正。
Nat Biotechnol. 2002 Jan;20(1):47-52. doi: 10.1038/nbt0102-47.
6
Fooling mother nature.
Nat Biotechnol. 2002 Jan;20(1):32-3. doi: 10.1038/nbt0102-32.
7
Evolutionary origin of SL-addition trans-splicing: still an enigma.
Trends Genet. 2001 Dec;17(12):678-80. doi: 10.1016/s0168-9525(01)02499-4.
8
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Gene. 2001 Oct 17;277(1-2):31-47. doi: 10.1016/s0378-1119(01)00695-3.
9
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