Jiang Yuehua, Henderson Dori, Blackstad Mark, Chen Angel, Miller Robert F, Verfaillie Catherine M
Stem Cell Institute, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
Proc Natl Acad Sci U S A. 2003 Sep 30;100 Suppl 1(Suppl 1):11854-60. doi: 10.1073/pnas.1834196100. Epub 2003 Aug 18.
We recently showed that a rare cell from murine bone marrow, which we termed multipotent adult progenitor cells (MAPCs), can be expanded for >120 population doublings. Mouse (m)MAPCs differentiate into mesenchymal lineage cells as well as endothelium and endoderm, and, when injected in the blastocyst, mMAPCs contribute to most if not all somatic cell lineages including the different cell types of the brain. Our results, reported herein, demonstrate that mMAPCs can also be induced to differentiate into cells having anatomical and electrophysiological characteristics similar to those of midbrain neurons. Differentiation to a neuronal phenotype was achieved by coculturing mMAPCs with astrocytes, suggesting that neuronal differentiation may require astrocyte-derived factors similar to what is required for the differentiation of embryonic stem cells and neural stem cells to neurons. Differentiation of mMAPCs to neuron-like cells follows similar developmental steps as described for embryonic stem cells and neural stem cells. MAPCs therefore may constitute a source of cells for treatment of central nervous system disorders.
我们最近发现，从小鼠骨髓中分离出的一种稀有细胞，我们将其命名为多能成体祖细胞（MAPCs），可以扩增超过120个群体倍增。小鼠（m）MAPCs可分化为间充质谱系细胞、内皮细胞和内胚层细胞，并且在注射到囊胚中时，mMAPCs可分化为大部分（如果不是全部）体细胞谱系，包括大脑的不同细胞类型。我们在此报告的结果表明，mMAPCs还可被诱导分化为具有与中脑神经元相似的解剖学和电生理特征的细胞。通过将mMAPCs与星形胶质细胞共培养实现了向神经元表型的分化，这表明神经元分化可能需要星形胶质细胞衍生的因子，类似于胚胎干细胞和神经干细胞向神经元分化所需的因子。mMAPCs向神经元样细胞的分化遵循与胚胎干细胞和神经干细胞相同的发育步骤。因此，MAPCs可能构成治疗中枢神经系统疾病的细胞来源。
