Mellin Hanna, Friesland Signe, Auer Gert, Dalianis Tina, Munck-Wikland Eva
Dept. of Oncology-Pathology, Cancer Center Karolinska, R8:01, Karolinska Institute, Karolinska Hospital, S-171 76 Stockholm, Sweden.
Anticancer Res. 2003 May-Jun;23(3C):2821-8.
Human papillomavirus (HPV) is frequently observed in tonsillar cancer. Here, tonsillar tumors were examined to see whether DNA content varied depending on HPV status, and influenced clinical outcome.
Image cytometry (ICM) was used for DNA content analysis and PCR for HPV analysis in 66 tonsillar cancer biopsies.
Most tumors exhibited a high degree of aneuploidy, while only 12% were diploid. Although non-significant, proportionally more patients with cancer with a 5c ER below the mean value compared to patients with tumors with a 5c ER above the mean value were disease-free and displayed a better survival. Forty-five percent of the tumors were HPV-16-positive. Significantly more HPV-positive tumors than HPV-negative tumors had a 5c ER below the mean value and more patients with HPV- positive, compared to patients with HPV-negative tumors stayed disease-free and had a better survival.
Tonsillar cancer displays a high degree of aneuploidy, albeit HPV-positive tumors have a lower degree of aneuploidy than HPV-negative tumors. Finally, HPV status provides a stronger prognostic value in tonsillar cancer than DNA content.
人乳头瘤病毒（HPV）在扁桃体癌中经常被发现。在此，对扁桃体肿瘤进行检查，以观察DNA含量是否因HPV状态而异，并影响临床结果。
对66例扁桃体癌活检组织采用图像细胞术（ICM）进行DNA含量分析，采用聚合酶链反应（PCR）进行HPV分析。
大多数肿瘤表现出高度的非整倍体性，而只有12%为二倍体。尽管差异不显著，但与5c ER高于平均值的肿瘤患者相比，5c ER低于平均值的癌症患者中无疾病生存且生存率更高的比例相对较高。45%的肿瘤为HPV-16阳性。HPV阳性肿瘤中5c ER低于平均值的比例明显高于HPV阴性肿瘤，与HPV阴性肿瘤患者相比，HPV阳性肿瘤患者无疾病生存且生存率更高。
扁桃体癌表现出高度的非整倍体性，尽管HPV阳性肿瘤的非整倍体程度低于HPV阴性肿瘤。最后，HPV状态在扁桃体癌中比DNA含量具有更强的预后价值。
