Martínez-Subiela Silvia, Bernal Luis J, Cerón José J
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, 30100 Espinardo, Murcia, Spain.
Am J Vet Res. 2003 Aug;64(8):1021-6. doi: 10.2460/ajvr.2003.64.1021.
To evaluate changes in serum concentrations of acute-phase proteins in dogs with leishmaniosis during short-term therapy in accordance with 2 treatment protocols and determine whether concentrations of acute-phase proteins could be used to monitor the initial response of dogs to treatment.
12 dogs naturally infected with Leishmania infantum.
Dogs were allocated into 2 groups. Dogs of group 1 were treated by use of meglumine antimonate (100 mg/kg, SC, q 24 h) administered concurrently with allopurinol (15 mg/kg, PO, q 12 h) for 20 days and then with allopurinol alone at the same dosage for the subsequent 30 days. Dogs of group 2 were treated by administration of allopurinol alone (15 mg/kg, PO, q 12 h) for 60 days). Blood samples were obtained before and during treatment for measurement of serum concentrations of acute-phase proteins and determination of CBC counts, serum biochemical analyses, and electropherograms.
All dogs evaluated in the study had increased concentrations of C-reactive protein, haptoglobin, and ceruloplasmin at the time of diagnosis of leishmaniosis. Mean concentration of serum amyloid A before treatment was also increased, but some of the dogs had concentrations of serum amyloid A that were within the reference range. Concentrations of C-reactive protein and ceruloplasmin decreased significantly in all dogs at the end of the study period.
Measurement of concentrations of selected acute-phase proteins, such as C-reactive protein or ceruloplasmin, could be used to evaluate the initial response of dogs with leishmaniosis to treatment.
根据两种治疗方案评估利什曼病犬短期治疗期间急性期蛋白血清浓度的变化，并确定急性期蛋白浓度是否可用于监测犬对治疗的初始反应。
12只自然感染婴儿利什曼原虫的犬。
将犬分为2组。第1组犬使用葡甲胺锑酸盐（100mg/kg，皮下注射，每24小时1次）与别嘌呤醇（15mg/kg，口服，每12小时1次）同时给药20天，然后在随后30天仅使用相同剂量的别嘌呤醇进行治疗。第2组犬仅使用别嘌呤醇（15mg/kg，口服，每12小时1次）治疗60天。在治疗前和治疗期间采集血样，以测量急性期蛋白的血清浓度，并进行全血细胞计数、血清生化分析和电泳图谱测定。
研究中评估的所有犬在诊断利什曼病时C反应蛋白、触珠蛋白和铜蓝蛋白的浓度均升高。治疗前血清淀粉样蛋白A的平均浓度也升高，但部分犬的血清淀粉样蛋白A浓度在参考范围内。在研究期结束时，所有犬的C反应蛋白和铜蓝蛋白浓度均显著下降。
测量选定的急性期蛋白浓度，如C反应蛋白或铜蓝蛋白，可用于评估利什曼病犬对治疗的初始反应。
