Flores Gema, Dastmalchi Keyvan, Paulino Sturlainny, Whalen Kathleen, Dabo Abdoulaye J, Reynertson Kurt A, Foronjy Robert F, D'Armiento Jeanine M, Kennelly Edward J
Department of Biological Sciences, Lehman College and The Graduate Center, City University of New York, 250 Bedford Park Boulevard West, Bronx, NY 10468, United States; Instituto de Fermentaciones Industriales, Consejo Superior de Investigaciones Científicas (CSIC), c/Juan de la Cierva 3, 28006 Madrid, Spain.
Department of Biological Sciences, Lehman College and The Graduate Center, City University of New York, 250 Bedford Park Boulevard West, Bronx, NY 10468, United States.
Food Chem. 2012 Oct 1;134(3):1256-62. doi: 10.1016/j.foodchem.2012.01.086. Epub 2012 Feb 18.
Nine anthocyanins (1-9) from the edible fruits of Eugenia brasiliensis were identified by HPLC-PDA and LC-MS, and seven of these are described for the first time in this Brazilian fruit. Two of the major anthocyanins, delphinidin (8) and cyanidin (9), were studied for their inhibitory activity against chemokine interleukin-8 (IL-8) production before and after cigarette smoke extract (CSE) treatment of cells. In non-treated cells the amount of IL-8 was unchanged following treatment with cyanidin and delphinidin in concentrations 0.1-10 μM. Both delphinidin (8) and cyanidin (9) decreased the production of IL-8 in treated cells, at 1 and 10 μM, respectively. Delphinidin (8) demonstrated IL-8 inhibition in the CSE treated cells in a dose-dependent manner.
通过高效液相色谱-光电二极管阵列检测法(HPLC-PDA)和液相色谱-质谱联用技术(LC-MS),从巴西番樱桃的可食用果实中鉴定出9种花色苷(1-9),其中7种是在这种巴西水果中首次被描述。研究了两种主要花色苷,飞燕草素(8)和矢车菊素(9)在细胞经香烟烟雾提取物(CSE)处理前后对趋化因子白细胞介素-8(IL-8)产生的抑制活性。在未处理的细胞中,用浓度为0.1-10μM的矢车菊素和飞燕草素处理后,IL-8的量没有变化。飞燕草素(8)和矢车菊素(9)分别在1μM和10μM时降低了经处理细胞中IL-8的产生。飞燕草素(8)在CSE处理的细胞中呈剂量依赖性地抑制IL-8。