Yamada Toshihiro, Ito Motomi, Kato Masahiro
Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Tokyo 113-0033, Japan.
Dev Genes Evol. 2003 Oct;213(10):510-3. doi: 10.1007/s00427-003-0350-8. Epub 2003 Aug 20.
Two homologues of INNER NO OUTER ( INO) in Nymphaea alba and N. colorata (Nymphaeaceae) were isolated and the expression pattern of the N. alba INO homologue NaINO was examined by in situ hybridization. The INO homologues obtained have a portion similar to INO in the predicted amino acid sequences between the conserved zinc finger-like and YABBY domains. In an in situ hybridization analysis, NaINO is expressed in the outer epidermis of the outer integument, inner integument, and the tip of the nucellus. The pattern observed in the outer integument is very similar to that of Arabidopsis thaliana, while the expression in the inner integument and nucellus is not observed in A. thaliana.
在白睡莲和彩色睡莲（睡莲科）中分离出了两个与“内无外”（INO）同源的基因，并通过原位杂交检测了白睡莲INO同源基因NaINO的表达模式。获得的INO同源基因在预测的氨基酸序列中，在保守的锌指样结构域和YABBY结构域之间有一部分与INO相似。在原位杂交分析中，NaINO在外珠被、内珠被的外表皮以及珠心顶端表达。在外珠被中观察到的表达模式与拟南芥非常相似，而在内珠被和珠心中的表达在拟南芥中未观察到。
