Zhang Xiaoqin, Yang Xianfeng, Zhang Qinqin, Wang Jialun, Zeng Tingting, Xi Yanan, Shen Qiang
Guizhou Tea Research Institute, Guiyang, 550006 China.
College of Tea Sciences, Guizhou University, Guiyang, 550025 China.
3 Biotech. 2024 Apr;14(4):113. doi: 10.1007/s13205-024-03940-9. Epub 2024 Mar 19.
The plant-specific transcription factor gene family, YABBY, plays an important role in plant development and stress response. Although genes have been identified in numerous species, a comprehensive characterization of s in tea tree and oil tea has been lacking. In this study, ten and three genes were identified in and , respectively. YABBY proteins could be divided into five subfamilies. Most genes in the same clade had similar structures and conserved motifs. Protein evolutionary analysis revealed that FIL/YAB3 displayed high conservation in all positions, followed by INO, YAB2, YAB5, and CRC. Specific site analysis suggested that the YABBY family was polyphyletic during the evolution. Compared to , two segmentally duplicated gene pairs were formed in during recent WGD events generated 30.69 and 45.08 Mya, respectively. -acting element indicated that most genes contain box4, ARE, and MYB elements. A total of 120 SSR loci were found within s, consisting of six types, while 48 SSR loci were identified within , consisting of three types. Transcriptome results revealed that CRC and INO clades were specifically expressed in floral organs. The expression of and was significantly up-regulated under drought and salt treatments, respectively, as confirmed by qRT-PCR. genes were more susceptible to salt stress, as increased by about 15-fold. Furthermore, functional differentiation may have occurred in duplicated genes. These discoveries provide important information for further research on s in tea tree and oil tea.
The online version contains supplementary material available at 10.1007/s13205-024-03940-9.
植物特异性转录因子基因家族YABBY在植物发育和胁迫响应中起重要作用。尽管已在许多物种中鉴定出该基因，但茶树和油茶中该基因家族的全面特征仍不清楚。在本研究中，分别在茶树和油茶中鉴定出10个和3个YABBY基因。YABBY蛋白可分为5个亚家族。同一进化枝中的大多数YABBY基因具有相似的结构和保守基序。蛋白质进化分析表明，FIL/YAB3在所有位置都表现出高度保守性，其次是INO、YAB2、YAB5和CRC。特定位点分析表明，YABBY家族在进化过程中是多系的。与茶树相比，油茶在分别于3069万年前和4508万年前发生的近期全基因组加倍事件中形成了两对片段重复基因对。顺式作用元件表明，大多数YABBY基因含有box4、ARE和MYB元件。在YABBY基因中总共发现了120个SSR位点，由6种类型组成，而在油茶的YABBY基因中鉴定出48个SSR位点，由3种类型组成。转录组结果显示，CRC和INO进化枝在花器官中特异性表达。qRT-PCR证实，茶树和油茶的YABBY基因在干旱和盐处理下分别显著上调。油茶的YABBY基因对盐胁迫更敏感，上调幅度约为15倍。此外，重复基因可能发生了功能分化。这些发现为进一步研究茶树和油茶中的YABBY基因提供了重要信息。
在线版本包含可在10.1007/s13205-024-03940-9获取的补充材料。
